草地贪夜蛾及二化螟肠道微生物多样性

Diversity of Gut Microbiota of Spodoptera Frugiperda and Chilo Suppressalis

采用高通量DNA测序技术以及微生物群落分析方法,对怒江下游蒲缥坝草地贪夜蛾、二化螟两种害虫肠道的微生物进行了研究。研究结果显示:单从菌群的Alpha多样性来看,两种昆虫肠道内微生物在物种数和均匀度方面基本相当;两组样本菌群在群落结构方面虽然存在差异,但不显著;在两组样本共有且相对丰度较高的10个属中,草地贪夜蛾中肠球菌属(Enterococcus)明显高于二化螟;而在二化螟中克雷伯氏菌属(Klebsiella)和气单胞菌属(Aeromonas)的丰度明显高于草地贪夜蛾。

High-throughput DNA sequencing and microbial community analysis were used to study the gut microbiota of Spodoptera frugiperda and Chilo suppressalis in the lower reaches of Nujiang River.The results showed that in terms of alpha diversity,the number of species and evenness of microorganisms in the gut of the two insects were basically the same,although there were differences in community structure between the two groups.Among the 10 genera shared and relatively abundant in the two groups,the Enterococcus in Spodoptera frugiperda was significantly higher than that in Chilo suppressa⁃lis.However,the abundance of Klebsiella and Aeromonas in Chilo suppressalis was significantly higher than that in Spodoptera frugiperda.