人参皂苷Rg3通过调控E2F1对人胃癌SGC-7901细胞生物行为学的影响

Effects of ginsenoside Rg3 on biobehavior of human gastric cancer SGC-7901 cells by regulating E2F1

目的探究人参皂苷Rg3通过调控E2F1对人胃癌SGC-7901细胞生物行为学的影响。方法MTT测定不同浓度人参皂苷Rg3(0、80、160、320μmol·L^(-1))对细胞增殖影响;流式细胞术测定不同浓度人参皂苷Rg3对细胞凋亡的影响;划痕愈合实验和Transwell实验测定不同浓度人参皂苷Rg3对细胞迁移及侵袭的影响;Western blot测定不同浓度人参皂苷Rg3对E2F1、MMP-2、MMP-9、BCL-2、Bax表达的影响。结果80、160、320μmol·L^(-1)人参皂苷Rg3组细胞存活率与空白对照组比较明显降低,且呈浓度依赖性(P<0.05)。80、160、320μmol·L^(-1)人参皂苷Rg3组细胞凋亡率与空白对照组比较明显增加,且呈浓度依赖性(P<0.05)。80、160、320μmol·L^(-1)人参皂苷Rg3组细胞迁移数目与空白对照组比较明显降低,且呈浓度依赖性(P<0.05)。80、160、320μmol·L^(-1)人参皂苷Rg3组细胞侵袭数目与空白对照组比较明显降低,且呈浓度依赖性(P<0.05)。80、160、320μmol·L^(-1)人参皂苷Rg3组E2F1 mRNA与E2F1蛋白表达量相较空白对照组明显减少且,呈浓度依赖性(P<0.05)。80、160、320μmol·L^(-1)人参皂苷Rg3组细胞中MMP-2、MMP-9、BCL-2蛋白表达量与空白对照组比较明显降低,BCL-2与空白对照组比较明显升高,且呈浓度依赖性(P<0.05)。结论人参皂苷Rg3能降低胃癌SGC-7901细胞增殖能力,抑制细胞迁移及侵袭能力,同时还促进SGC-7901细胞凋亡,且具有浓度依赖性,其作用机制可能通过E2F1因子下调MMP-2、MMP-9、BCL-2表达,上调Bax表达有关。

Aim To explore the effect of ginsenoside Rg3 on the biological behavior of human gastric cancer SGC-7901 cells by regulating E2F1.Methods MTT assay was used to determine the effect of ginsenoside Rg3(0,80,160,320μmol·L^(-1))on cell proliferation.The effects of different concentrations of ginsenoside Rg3 on apoptosis were measured by flow cytometry.The effects of different concentrations of ginsenoside Rg3 on cell migration and invasion were determined by scratch healing experiment and Transwell experiment.The effects of different concentrations of ginsenoside Rg3 on the expression of E2F1,MMP-2,MMP-9,BCL-2 and Bax were determined by Western blot.Results Compared with the blank control group,the cell survival rate of 80,160 and 320μmol·L^(-1) ginsenoside Rg3 group was significantly lower,and it was concentration-dependent(P<0.05).Compared with the blank control group,the apoptosis rate of 80,160 and 320μmol·L^(-1) ginsenoside Rg3 group significantly increased in a concentration-dependent manner(P<0.05).Compared with the blank control group,the number of cell migration in 80,160 and 320μmol·L^(-1) ginsenoside Rg3 groups was significantly lower in a concentration-dependent manner(P<0.05).Compared with the blank control group,the number of cell invasion in 80,160 and 320μmol·L^(-1) ginsenoside Rg3 groups was significantly lower in a concentration-dependent manner(P<0.05).The E2F1 mRNA and E2F1 protein expression in the 80,160,and 320μmol·L^(-1) ginsenoside Rg3 groups were significantly reduced in a concentration-dependent manner compared with that in the blank control group(P<0.05).The protein expression of MMP-2,MMP-9,and BCL-2 in the cells of 80,160,and 320μmol·L^(-1) ginsenoside Rg3 group significantly decreased compared with those of the blank control group,and BCL-2 significantly increased compared with that of the blank control group in a concentration-dependent manner(P<0.05).Conclusions Ginsenoside Rg3 can reduce the proliferation,inhibit the migration and invasion of gastric cancer SGC-7901 cells,and promote the apoptosis of SGC-7901 cells in a concentration-dependent manner,and its mechanism may be related to the down-regulation of MMP-2,MMP-9,and BCL-2 expression and up-regulation of Bax expression through E2F1.