肝细胞癌患者血清ECM1、MMP-9和VEGF水平的变化及其意义

Changes of serum ECM1,MMP-9 and VEGF levels in patients with hepatocellular carcinoma and their significance

目的 探究肝细胞癌患者血清细胞外基质蛋白-1(ECM1)、基质金属蛋白酶-9(MMP-9和血管内皮生长因子(VEGF)水平的变化及其意义。方法 对60例肝细胞癌患者进行回顾性分析,患者主要使用甲磺酸阿帕替尼进行治疗,必要时也可联合GEMOX方案肝动脉化疗栓塞术进行治疗。采用酶联免疫吸附法测定患者治疗前后的血清ECM1、VEGF、MMP-9水平。比较患者治疗前后ECM1、MMP-9和VEGF水平;比较不同临床病理特征(年龄、性别、血管侵犯、淋巴结转移、TNM分期、Edmondson分期和肿瘤直径)患者ECM1、MMP-9和VEGF水平。结果 治疗后,患者ECM1、MMP-9以及VEGF水平分别为(135.23±44.58)pg/ml、(421.25±87.56)μg/L和(657.56±54.56)pg/ml,均低于治疗前的(215.56±30.80)pg/ml、(499.56±30.56)μg/L、(798.02±50.79)pg/ml(P<0.05)。不同年龄、性别患者ECM1、MMP-9、VEGF水平比较差异无统计学意义(P>0.05);不同血管侵犯、淋巴结转移、TNM分期、Edmondson分期和肿瘤直径患者ECM1、MMP-9、VEGF水平比较差异具有统计学意义(P<0.05)。结论 ECM1、MMP-9、VEGF三者相互作用可促进肝癌细胞的转移,可根据其水平情况,了解患者肝细胞、肝功能健康状况。

Objective To investigate the changes of serum extracellular matrix protein 1(ECM1),matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF)levels in patients with hepatocellular carcinoma(HCC)and their significance.Methods A total of 60 patients with hepatocellular carcinoma were retrospectively analyzed.Patients were mainly treated with apatinib mesylate,and can also be treated by transcatheter arterial chemoembolization with GEMOX regimen if necessary.The serum ECM1,VEGF and MMP-9 levels were measured by enzyme linked immunosorbent assay before and after treatment.The levels of ECM1,MMP-9 and VEGF were compared before and after treatment.The levels of ECM1,MMP-9 and VEGF were compared among patients with different clinicopathologic features(age,gender,vascular invasion,lymph node metastasis,TNM stage,Edmondson stage and tumor diameter).Results After treatment,the levels of ECM1,MMP-9 and VEGF were(135.23±44.58)pg/ml,(421.25±87.56)μg/L and(657.56±54.56)pg/ml,which were lower than(215.56±30.80)pg/ml,(499.56±30.56)μg/L and(798.02±50.79)pg/ml before treatment(P<0.05).There were no significant differences in the levels of ECM1,MMP-9 and VEGF among patients of different ages and genders(P>0.05).There were statistical significant differences in ECM1,MMP-9 and VEGF levels among patients with different vascular invasion,lymph node metastasis,TNM stage,Edmondson stage and tumor diameter(P<0.05).Conclusion The interaction of ECM1,MMP-9 and VEGF can promote the metastasis of liver cancer cells,and the health status of liver cells in patients can be understood according to the level.