期刊文献+

辣木籽水提物对5-氟尿嘧啶诱导小鼠空肠黏膜炎的调节作用

Regulatory Effect of Moringa oleifera Seeds Water Extract on Jejunal Mucositis Induced by 5-Fluorouracil in Mice
下载PDF
导出
摘要 【目的】探究辣木籽水提物对5-氟尿嘧啶(5-fluorouracil,5-FU)诱导引起的小鼠空肠黏膜炎的改善作用。【方法】将50只SPF级雄性昆明小鼠随机分为正常组、模型组(腹腔注射5-FU 130 mg/kg)及辣木籽水提物低、中、高剂量组(分别灌胃辣木籽水提物50、100和200 mg/kg)。第1~14天,每日正常组和模型组灌胃蒸馏水,辣木籽组灌胃不同剂量的辣木籽水提物;第15~16天,每日正常组腹腔注射生理盐水,其余组均腹腔注射5-FU;第17~18天观察;第19天处死小鼠。每日记录小鼠摄食量、饮水量及体质量;采用HE染色并观察小鼠空肠组织形态学变化;采用免疫组化法检测空肠增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达情况;采用Tunel法检测空肠细胞的凋亡情况;采用酶联免疫法(ELISA)测定空肠组织IL-6、IL-1β和TNF-α炎症因子的含量变化。【结果】与模型组相比,辣木籽水提物高剂量组(200 mg/kg)可显著缓解小鼠体质量下降,极显著提高空肠绒毛高度、降低隐窝深度、增加绒毛高度与隐窝深度的比值,提高空肠PCNA表达量,降低细胞凋亡程度,抑制IL-6、IL-1β和TNF-α的表达。【结论】辣木籽水提物可改善5-FU诱导引起的小鼠空肠黏膜炎,且呈剂量依赖性。 [Purpose]To explore the improvement of Moringa oleiferia seeds water extract on jejunal mucositis induced by 5-fluorouracil(5-FU)in mice.[Methods]A total of 50 SPF grade male Kunming mice were randomly divided into normal group,model group(intraperitoneally injec-ted with 130 mg/kg 5-FU)and low dose,medium dose and high dose of M.oleiferia seeds water extract groups(administrated with M.oleiferia seeds water extract 50,100 and 200 mg/kg,respectively).From the 1st day to the 14th day of the experiment,normal group and model group were given distilled water,M.oleiferia seeds group was given different doses of M.oleiferia seed water extract every day;from the 15th day to the 16th day,normal group was intraperitoneally injected with normal saline,and other groups were intraperitoneally injected with 5-FU every day;observation was carried out from the 17th day to the 18th day;the mice were killed on the 19th day.Food intake,water intake and body weight of mice were recorded daily;the histopathological changes of jejunum were observed by HE staining;the expression of proliferating cell nuclear antigen(PCNA)in jejunum was observed by immunohistochemistry;the apoptosis of jejunum cell was detected by Tunel method;the contents of IL-6,IL-1βand TNF-αin jejunum tissue were determined by enzyme-linked immunoassay(ELISA).[Results]Compared with model group,high dose of M.oleiferia seeds water extract group(200 mg/kg)could alleviate the weight loss of mice significantly;extremely significantly increase the villous length of jejunum,decrease the crypt depth,and increase the ratio of villous length to crypt depth;increase the expression of PCNA in jejunum;reduce the degree of apoptosis;inhibite the expression of IL-6,IL-1βand TNF-α.[Conclusion]M.oleifonica seeds water extract can ameliorate 5-FU-induced jejunal mucositis,and with a dose-dependent manner.
作者 赵润萍 黄开兰 宋爽 白忠彬 ZHAO Runping;HUANG Kailan;SONG Shuang;BAI Zhongbin(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;National Research and Development Center of Moringa oleifera Processing Technology,Kunming 650201,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China)
出处 《云南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期43-52,共10页 Journal of Yunnan Agricultural University:Natural Science
基金 国家自然科学基金项目(31960508)。
关键词 辣木籽水提物 5-氟尿嘧啶 空肠黏膜炎 调节作用 Moringa oleifera seeds water extract 5-fluorouracil jejunal mucositis regulatory effect
  • 相关文献

参考文献8

二级参考文献86

共引文献226

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部