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5-氨基酮戊酸光动力治疗对鲍温病皮损中p53 Caveolin-1表达的影响

Influence of 5-Aminolevulinic Acid Photodynamic Therapy on the Expression of p53 and Caveolin-1 in Bowen's Disease Lesions
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摘要 目的:探讨5-氨基酮戊酸光动力疗法对鲍温病皮损中p53、Caveolin-1表达的影响及意义。方法:运用5-氨基酮戊酸光动力疗法治疗鲍温病及周围正常皮肤组织各40例,采用免疫组织化学技术(SP法)检测光动力治疗前后鲍温病及周围正常皮肤组织中p53、Caveolin-1的阳性细胞表达率。结果:治疗前p53蛋白在正常皮肤组织及BD中表达的阳性率分别为10%、40%(χ^(2)=11.202,P<0.001),差异有统计学意义。治疗后p53在正常皮肤组织及BD的阳性率为10%、5%(χ^(2)=0.712,P=0.399),两者间差异无统计学意义。治疗前后p53在BD中的阳性表达率差异有统计学意义(χ^(2)=14.811,P<0.001)。治疗前Caveolin-1在正常皮肤组织及BD中阳性表达率为15%、55%(χ^(2)=14.449,P<0.001),两者间差异有统计学意义。治疗后正常皮肤组织及BD中Caveolin-1的阳性率为5%、12.5%(χ^(2)=0.816,P=0.366),差异无统计学意义。治疗前后Caveolin-1在BD中的阳性表达率差异有统计学意义(χ^(2)=19.013,P<0.001)。BD中p53与Caveolin-1阳性表达呈正相关性(r=0.533,P=0.015)。结论:p53及Caveolin-1的高表达可能与BD的发生密切关联,且ALA-PDT能够抑制p53及Caveolin-1的表达,从而抑制疾病的发展。通过更大样本量的研究,p53及Caveolin-1可能会成为皮肤相关疾病的诊断工具,并为其治疗提供新的靶点。 Objective:To investigate the influence and significance of 5-aminolevulinic acid photodynamic therapy(ALA-PDT)on the expression of p53 and Caveolin-1 in Bowen's disease(BD)lesions.Methods:Forty cases of Bowen's disease and adjacent normal skin tissues were treated with ALA-PDT.Immunohistochemical staining(SP method)was used to detect the positive expression rates of p53 and Caveolin-1 in BD and surrounding normal skin tissues before and after photodynamic therapy.Results:Before treatment,the positive rates of p53 protein expression in normal skin tissue and BD were 10%and 40%,respectively(χ^(2)=11.202,P<0.001),with a statistically significant difference.After treatment,the positive rates of p53 in normal skin tissue and BD were 10%and 5%(χ^(2)=0.712,P=0.399),respectively,with no statistically significant difference between them.The difference in the positive expression rate of p53 in BD before and after treatment was statistically significant(χ^(2)=14.811,P<0.001).Before treatment,the positive expression rates of Caveolin-1 in normal skin tissue and BD were 15%and 55%,respectively(χ^(2)=14.449,P<0.001),with a statistically significant difference.After treatment,the positive rates of Caveolin-1 in normal skin tissue and BD were 5%and 12.5%(χ^(2)=0.816,P=0.366),respectively,with no statistically significant difference between them.The difference in the positive expression rate of Caveolin-1 in BD before and after treatment was statistically significant(χ^(2)=19.013,P<0.001).There was a positive correlation between p53 and Caveolin-1 expression in BD(r=0.533,P=0.015).Conclusion:The high expression of p53 and Caveolin-1 may be closely related to the occurrence of BD,and ALA-PDT can inhibit the expression of p53 and Caveolin-1,thereby inhibiting the progression of the disease.With a larger sample size,p53 and Caveolin-1 may become diagnostic tools for skin-related diseases and provide new targets for their treatment.
作者 张艳峰 樊磊强 高悦 孙业晓 徐冰 ZHANG Yanfeng;FAN Leiqiang;GAO Yue(Chengde Central Hospital,Hebei Chengde 067000,China)
出处 《河北医学》 CAS 2024年第5期794-798,共5页 Hebei Medicine
基金 河北省承德市科学技术研究与发展计划项目,(编号:202002A006)。
关键词 5-氨基酮戊酸光动力疗法 鲍温病 P53 CAVEOLIN-1 5-aminoketovaleric acid photodynamic therapy Bowen's disease p53 Caveolin-1
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