大血藤多酚组分的化学表征及抗氧化活性研究

Study on chemical characterization and antioxidant activity of polyphenols from Sargentodoxa cuneata

本实验富集大血藤多酚组分并对其进行含量测定和化学表征,丰富该药材的物质研究基础,初步分析多酚化合物体外抗氧化活性作用,为后续研究提供理论基础。采用紫外可见分光光度计确定最大吸收波长并测定总多酚含量,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱仪对其进行化学表征,利用DPPH法和ABTS法评价大血藤总多酚的体外抗氧化活性。结果显示优化Folin-Ciocalte法最大吸收波长为740 nm,大血藤总多酚含量约为32.34%;通过高分辨质谱检测到大血藤中多酚类成分有62个,包括28个酚酸和酚苷类成分、13个木脂素类成分、12个苯丙素类成分、9个黄酮类成分;提取物对DPPH和ABTS自由基都具有清除作用,IC50值分别为4.88μg/mL、16.68μg/mL。该研究从大血藤提取物中检测到62个多酚类成分,抗氧化活性与其密切相关,深入研究了大血藤多酚组分,为该药的进一步发展提供理论基础。

The polyphenol components of Sargentodoxa cuneata were enriched and their content determination and chemical characterization were carried out to enrich the material research basis of the medicinal material,and the antioxidant activity of polyphenol compounds in vitro was preliminarily analyzed to provide a theoretical basis for subsequent research.The maximum absorption wavelength was determined by ultraviolet-visible spectrophotometer and the total polyphenol content was determined.The chemical characterization was performed by ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometer.The antioxidant activity of total polyphenols in Sargentodoxa cuneata was evaluated by DPPH and ABTS methods.The maximum absorption wavelength of the optimized Folin-Ciocalte method was 740 nm,and the results showed that the total polyphenol content of Sargentodoxa cuneata was about 32.34%.By high resolution mass spectrometry,62 polyphenols were detected in Sargentodoxa cuneata,including 28 phenolic acids and phenolic glycosides,13 lignans,12 phenylpropanoids,and 9 flavonoids.The extract had scavenging effects on DPPH and ABTS free radicals,and the IC50 values were 4.88μg/mL and 16.68μg/mL,respectively.62 polyphenols were detected from the extract of Sargentodoxa cuneata,and the antioxidant activity was closely related to it.This experiment deeply studied the components of Sargentodoxa cuneata and provided a theoretical basis for the further development of the drug.