过表达Msx2促进小鼠胚胎干细胞向滋养层干细胞命运转变

Overexpression of Msx2 Promoted the Transition of Mouse Embryonic Stem Cells to Trophoblast Stem Cells

第一次细胞命运决定中相同的卵裂球逐渐分化形成内细胞团(inner cell mass,ICM)和滋养外胚层(trophectoderm,TE)两个不同的谱系,这一过程的正确进行是小鼠胚胎正常发育的必要条件.然而小鼠的ICM与TE尽管最初来源于相同的卵裂球,却有着不可逾越的屏障,自然情况下ICM与TE两个谱系之间不能相互转变.在小鼠胚胎干细胞(embryonic stem cells,ESCs)中利用piggyBac(PB)转座子系统过表达基因Msx2,并将Msx2过表达的小鼠ESCs培养在滋养层干细胞(trophoblast stem cells,TSCs)的条件培养基中进行诱导,可以使小鼠ESCs转变为TSCs,这一结果为研究第一次细胞命运决定的潜在机制提供了理论依据.

In the first cell fate decision,identical blastomere gradually differentiate into inner cell mass(ICM)and trophectoderm(TE)two different cell lineages.However,in mice although ICM and TE originally derived from the same blastomere,they have an impassable barrier,and the lineages of ICM and TE cannot convert to each other under natural conditions.In this study,piggyBac transposons were used to construct an overexpression(OE)system of Msx2 in mouse embryonic stem cells(ESCs).And Msx2-OE mESCs were induced in the conditioned medium(CM)of trophoblast stem cells(TSCs),which can be induced transit from mESCs to mTSCs.This result provides a reference for studying the potential mechanism of the first cell fate decision.