同位素内标稀释-液相色谱-串联质谱法测定化妆品中大麻二酚和四氢大麻酚的含量

Determination of Cannabidiol and Tetrahydrocannabinol in Cosmetics by Liquid Chromatography Tandem Mass Spectrometry with Isotope Internal Standard Dilution Technique

采用液相色谱-串联质谱法(LC-MS/MS)联用检测技术和内标标准曲线法定量方式,建立了化妆品中大麻二酚(CBD)和四氢大麻酚(THC)的检测方法。样品采用加入内标后经甲醇萃取,稀释定容,通过高效液相色谱分离,串联质谱定性和定量检测,由C18柱(100 mm×2.1 mm×3μm)分离,多反应监测(MRM)检测,内标标准曲线法定量。结果发现,CBD和THC的线性范围为0.5~50μg/L,相关系数≥0.999,方法的检出限为5μg/kg,定量限为10μg/kg。CBD和THC的平均回收率为83.8%~112.3%,相对标准偏差(RSD)<10.0%。该方法稳定、可靠,样品前处理简单,耗时短,同时具备较高的灵敏度和准确性。在化妆品监管中,填补了CBD和THC准确定性和定量分析的空白,适用于化妆品中CBD和THC的快速筛查。

A method for the determination of cannabidiol(CBD)and tetrahydrocannabinol(THC)in cosmetics was established by using by a detection technology of liquid chromatography tandem mass spectrometry(LC-MS/MS)and quantitative method using internal standard curve method.The sample was extracted with methanol after adding internal standards,diluted to volume,separated by high-performance liquid chromatography,and qualitatively and quantitatively detected by tandem mass spectrometry.They were separated by C18 column(100 mm×2.1 mm×3μm).The multi reaction monitoring(MRM)mode was performed and the internal standard curve method was used for quantitative analysis.The results showed that in this method,the linear range of CBD and THC was 0.5-50μg/L,the correlation coefficient was≥0.999,the detection limit was 5μg/kg,the quantitative limit was 10μg/kg.The average recoveries of CBD and THC ranged from 83.8% to 112.3%,and the relative standard deviation(RSD)was less than 10.0%.The method is stable,reliable,simple sample pretreatment,short analysis time,good sensitivity and accuracy.In the regulation of cosmetics,the gap in accurate qualitative and quantitative analysis of CBD and THC has been filled.And it is suitable for the rapid screening of CBD and THC in cosmetics.