摘要
建立了一种同位素稀释-气相色谱-质谱法测定血清中三种支链氨基酸含量的检测方法。采用该方法对血清中缬氨酸、亮氨酸、异亮氨酸进行定量分析,结果的相对标准偏差为0.19%~0.46%,平均回收率为99.9%~102.2%。方法重复性好、准确度高、结果准确可靠,可满足临床血清样品中三种氨基酸含量的准确检测。
An isotope dilution-gas chromatography-mass spectrometry method for the determination of three branched chain amino acids in serum was established.The established quantitative analysis method was used to analyze valine,leucine and isoleucine in serum.The relative standard deviation of the results was 0.19%-0.46%,and the average recovery was 99.9%-102.2%.The method has good repeatability,high accuracy and reliability,which could be used for accurate detection of three branched chain amino acids in clinical serum samples.
作者
朱天翼
孟娇然
夏娃
杨柳
商易安
Zhu Tianyi;Meng Jiaoran;Xia Wa;Yang Liu;Shang Yian(Shanghai Institute of Measurement and Testing Technology)
出处
《上海计量测试》
2024年第3期22-26,共5页
Shanghai Measurement and Testing
基金
上海市市场监督管理局科技项目(2023-12)。
关键词
血清
同位素稀释
支链氨基酸
气相色谱-质谱法
serum
isotope dilution
branched chain amino acids
gas chromatography-mass spectrometry
