中华绒螯蟹MIH基因SNP位点筛选及其与生长性状的关联分析

Screening of SNP loci of MIH gene in Eriocheir sinensis and its association with growth traits

为明确蜕皮抑制激素基因(MIH)对中华绒螯蟹的生长调控机制,本研究选取100只中华绒螯蟹幼蟹个体,分析幼蟹MIH基因的单核苷酸多态性(SNP)位点及基因型,并对与生长指标相关的多态性位点进行连锁不平衡和单倍型分析,进一步明确多态性位点单倍型与生长性状之间的相关性。结果显示,在中华绒螯蟹幼蟹MIH基因中共筛选鉴定出5个SNP位点,其中3个位点(C640G、C2529T和G2595T)与中华绒螯蟹生长性状具有相关性;3个相关位点中共检测到5种单倍型,其中H1单倍型(GCG)的占比最高(68.8%),为优势单倍型;H3单倍型(GTT)个体的生长性状指标最高,显著高于H2单倍型(CCG)。本研究得到的中华绒螯蟹MIH基因上3个与生长性状相关的SNP位点,可作为候选分子标记用于中华绒螯蟹优质品种选育。

In order to clarify the regulatory mechanism of molt-inhibiting hormone(MIH)gene on the growth of Chinese mitten crab(Eriocheir sinensis),100 juvenile Chinese mitten crabs were selected to analyze the single nucleotide polymorphism(SNP)loci and genotypes of MIH gene,and the linkage disequilibrium and haplotype analysis of polymorphic loci related to growth indicators were carried out to further clarify the correlation between haplotypes of polymorphic loci and growth traits.The results showed that five SNP loci were screened and identified in MIH gene of juvenile Chinese mitten crab,and three of them(C640G,C2529T and G2595T)were related to the growth traits of Chinese mitten crab.Five haplotypes were detected in the three related sites.Among them,H1 haplotype(GCG)accounted for the highest proportion(68.8%),which was the dominant haplotype.The growth traits of individuals with H3 haplotype(GTT)were the highest and significantly higher than those of individuals with H2 haplotype(CCG).The three SNP loci associated with growth traits in MIH gene of Chinese mitten crab obtained in this study can be used as candidate molecular markers for the breeding of high-quality Chinese mitten crab varieties.