白细胞介素10基因多态性和多囊卵巢综合征遗传易感性的研究

A study on the interleukin-10 gene polymorphism and genetic susceptibility to polycystic ovary syndrome

目的 探讨白细胞介素10(interleukin-10,IL-10)基因rs1800871 A/G的单核苷酸多态性(single nucleotide polymorphism, SNP)和rs1800872 T/G SNP与多囊卵巢综合征(polycystic ovary syndrome, PCOS)患者遗传易感性。方法 通过SNaPshot SNP分型方法共分析了121例PCOS患者和158例健康对照IL-10基因rs1800871 A/G和rs1800872 T/G的单核苷酸多态性。结果 病例组体重指数、肥胖家族史阳性、T水平高于对照组,FSH水平低于健康对照组(P<0.05)。采用SHEsis软件对这两个多态性位点进行分析显示,两个位点存在完全连锁现象(D'=1)。病例组与健康对照组基因型构成和等位基因差异有统计学意义(P<0.05)。Logistic回归分析结果显示,肥胖家族史、IL-10基因rs1800871A/G单核苷酸多态性是PCOS发病的危险因素(P<0.05)。结论 IL-10基因rs1800871 A/G增加了对PCOS的遗传易感性,携带AG和GG基因型可能增加了PCOS的发病风险。

Objective To explore interleukin-10(IL-10)gene rs1800871 A/G single nucleotide polymorphism(SNP)and rs1800872 T/G SNP and genetic susceptibility to polycystic ovary syndrome(PCOS).Methods The SNP of IL-10 gene rs1800871 A/G and rs1800872 T/G were analyzed by SNaPshot SNP typing method in 121 PCOS patients and 158 healthy controls.Results The level of body mass index,family history of obesity and T level in case group were higher than those in control group,while FSH level was lower than that in healthy control group(P<0.05).Polymorphic loci of Rs1800871 A/G SNP and rs1800872 T/G SNP of IL-10 were analysed by using SHEsis software,and results showed that complete linkage exists between these two SNPs(D'=1).There were significant differences in genotype composition and alleles between the case group and the healthy control group(P<0.05).Logistic regression analysis showed that family history of obesity and rs1800871A/G SNPof IL-10 gene were risk factors for PCOS(P<0.05).Conclusion The IL-10 rs1800871 A/G increases genetic susceptibility to PCOS,and carrying AG and GG genotypes may increase the risk of PCOS.