摘要
Signifcant advancements have been made in recent years in the development of highly sophisticated skin organoids.Serving as three-dimensional(3D)models that mimic human skin,these organoids have evolved into complex structures and are increasingly recognized as efective alternatives to traditional culture models and human skin due to their ability to overcome the limitations of two-dimensional(2D)systems and ethical concerns.The inherent plasticity of skin organoids allows for their construction into physiological and pathological models,enabling the study of skin development and dynamic changes.This review provides an overview of the pivotal work in the progression from 3D layered epidermis to cyst-like skin organoids with appendages.Furthermore,it highlights the latest advancements in organoid construction facilitated by state-of-the-art engineering techniques,such as 3D printing and microfuidic devices.The review also summarizes and discusses the diverse applications of skin organoids in developmental biology,disease modelling,regenerative medicine,and personalized medicine,while considering their prospects and limitations.
基金
suppor ted by the National Key Research and Development Program of China(2022YFA1104800)
the Beijing Nova Program(20220484100)
the National Natural Science Foundation of China(81873939)
the Open Research Fund of State Key Laboratory of Cardiovascular Disease,Fuwai Hospital(2022KF-04)
the Clinical Medicine Plus X-Young Scholars Projec t,Pek ing Universit y(PKU2022LCXQ003)
the Emerging Engineering InterdisciplinaryYoung Scholars Project,Peking University,the Fundamental Research Funds for the Central Universities(PKU2023XGK011)
the Open Research Fund of State Key Laboratory of Digital Medical Engineering,Southeast University(2023K-01)
the Open Research Fund of Beijing Key Laboratory of Metabolic Disorder Related Cardiovascular Disease,Beijing,China(DXWL2023-01)
the Science and Technology Bureau Foundation Application Project of Changzhou(CJ20220118)。
