LncRNA ZEB1-AS1和LncRNA SOX2OT在糖尿病肾病患者中的表达及与肾功能的相关性研究

Expression of LncRNA ZEB1-AS1 and LncRNA SOX2OT in patients with diabetic nephropathy and their correlation with renal function

目的探究长链非编码RNA锌指E盒结合同源盒蛋白1反义链1(LncRNA ZEB1-AS1)和长链非编码RNA性别决定相关基因簇2重叠转录本(LncRNA SOX2OT)在糖尿病肾病(DN)患者中的表达及与肾功能的相关性。方法选取于2021年11月—2023年12月在武汉大学人民医院肾内科收治的DN患者106例为DN组,并根据24 h尿蛋白定量(24 h Upro)水平分为正常蛋白尿亚组43例(<30 mg)、微量蛋白尿亚组39例(30~<300 mg)、大量蛋白尿亚组24例(≥300 mg),另选取同期医院单纯糖尿病患者106例作对照组,检测患者血清LncRNA ZEB1-AS1、LncRNA SOX2OT水平;Pearson法分析LncRNA ZEB1-AS1和LncRNA SOX2OT与肾功能指标的相关性;Logistic分析影响DN患者肾功能损伤的因素。结果DN组血清LncRNA ZEB1-AS1、LncRNA SOX2OT水平低于对照组(t=11.471、10.257,P均<0.001)。血清LncRNA ZEB1-AS1、LncRNA SOX2OT比较,正常尿蛋白亚组>微量尿蛋白亚组>大量尿蛋白亚组(F=58.720、117.722,P均<0.001),BUN、SCr、UA水平比较,正常尿蛋白亚组<微量尿蛋白亚组<大量尿蛋白亚组,差异均有统计学意义(F=122.493、595.589、53.178,P均<0.001);LncRNA ZEB1-AS1、LncRNA SOX2OT分别与BUN、SCr、UA呈负相关(r=-0.487、-0.498、-0.521,-0.527、-0.515、-0.534,P均<0.001);Logistic回归分析显示,糖尿病病程长及高BUN、SCr、UA水平是影响DN患者肾功能损伤的危险因素[OR(95%CI)=1.672(1.128~2.479)、2.839(1.534~5.253)、2.754(1.512~5.017)、2.693(1.464~4.954)],高LncRNA ZEB1-AS1、LncRNA SOX2OT是保护因素[OR(95%CI)=0.875(0.798~0.959)、0.898(0.832~0.969)]。结论血清LncRNA ZEB1-AS1、LncRNA SOX2OT水平与DN患者肾功能有关,可能是评估DN患者肾功能的潜在指标。

Objective To investigate the expression of long non-coding RNA zinc finger E-box binding homeobox protein 1 antisense chain 1(LncRNA ZEB1-AS1)and long non-coding RNA SOX2 overlapping transcript(LncRNA SOX2OT)in patients with diabetic nephropathy(DN)and their correlation with renal function.Methods In this study,106 patients with DN admitted in Department of Nephrology,Renmin Hospital of Wuhan University from November 2021 to December 2023 were collected as the DN group,and 106 patients with simple diabetes in the hospital were regarded as the control group.Both groups were collected venous blood.The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT were detected.According to the renal function of DN patients,they were separated into groups.The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT,and renal function,blood lipids,and blood glucose levels were compared.Pearson method was applied to analyze the correlation between LncRNA ZEB1-AS1 and LncRNA SOX2OT with renal function indicators.Logistic was applied to analyze the factors affecting renal function impairment in DN patients.Results The serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT in the DN group were lower than those in the control group(t=11.471,10.257/all P<0.001).The levels of serum LncRNA ZEB1-AS1 and LncRNA SOX2OT in the normal urine protein subgroup were higher than those in the microalbuminuria subgroup and higher than those in the large urine protein subgroup(F=58.720,117.722,122.493,595.589,53.178/all P<0.001).The levels of serum BUN,SCr,UA in the normal urine protein subgroup were lower than those in the microalbuminuria subgroup and lower than those in the large urine protein subgroup(F=122.493,595.589,53.178,all P<0.001).LncRNA ZEB1-AS1 was negatively correlated with BUN,SCr,UA(r=-0.487,-0.498,-0.521,P<0.001),there was a negative correlation between LncRNA SOX2OT and the above indicators(r=-0.527,-0.515,-0.534,P<0.001).Logistic model analysis showed that the course of diabetes,BUN,SCr and UA levels were risk factors for renal function damage in DN patients[OR(95%CI)=1.672(1.128-2.479),2.839(1.534-5.253),2.754(1.512-5.017),2.693(1.464-4.954)],and high LncRNA ZEB1-AS1 and high LncRNA SOX2OT were protective factors[OR(95%CI)=0.875(0.798-0.959),0.898(0.832-0.969)].Conclusion Serum levels of LncRNA ZEB1-AS1 and LncRNA SOX2OT are related to renal function in DN patients,and may be potential indicators for evaluating renal function in DN patients.