基于非靶向代谢组学分析两种酵母培养物的成分差异

Analysis of the composition differences between two yeast cultures based on untargeted metabolomics

【目的】比较两种酵母培养物及其代谢成分的差异,为指导酵母培养物的生产及应用提供参考。【方法】材料为1种自研酵母培养物与达农威益康XP酵母培养物,利用非靶标代谢组学UHPLC-QTOF-MS技术,分析比较二者代谢产物成分及差异。【结果】(1)在二级类别注释下,正、负离子模式分别注释到614、497个化合物,主要代谢类别为有机酸,核苷、核苷酸及其衍生物,氨基酸及其衍生物,两种酵母培养物均无特有代谢成分,只在含量上有显著(P<0.05)差异。(2)共237个差异代谢物在正离子模式检出,176个显著(P<0.05)上调表达,61个显著(P<0.05)下调表达;136个差异代谢物在负离子模式检出,64个显著(P<0.05)上调表达,72个显著(P<0.05)下调表达。(3)差异代谢物的KEGG(Kyoto Encyclopedia of Genes and Genomes)富集分析主要集中在丙氨酸、天冬氨酸和谷氨酸等代谢通路。【结论】自研酵母培养物与达农威益康XP的代谢产物含量存在显著差异,但其发酵原料及工艺更简便,并含有多种具药理、生理作用的代谢成分,有潜在应用价值。

【Objective】This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture.【Methods】The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures.【Results】(1)Under the super classification annotation,a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids,nucleosides,nucleotides and their derivatives,amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures,only significant differences in content(P<0.05).(2)In the positive ion mode,237 differential metabolites were detected,of which 176 were significantly up-regulated(P<0.05)and 61 were significantly down-regulated(P<0.05)in expression.In the negative ion mode,136 differential metabolites were detected,of which 64 were significantly up-regulated(P<0.05)and 72 were significantly down-regulated(P<0.05)in expression.(3)The KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment pathway of differential metabolites mainly focused on alanine,aspartate and glutamate metabolism.【Conclusion】Due to different carbon and nitrogen sources and fermentation conditions,the yeast cultures have significant differences in the content of metabolites,but with their own advantages of the biological activity of main differential metabolites.