杜仲叶际可培养真菌群落组成及产IAA菌株筛选

Community Composition of Culturable Fungi in Eucommia Ulmoides Phyllosphere and Screening of IAA-Producing Strains

杜仲作为我国特有、贵州广泛分布的药用植物,其叶际微生物对其生长具有重要意义。为揭示人工栽培和野生杜仲叶际真菌群落组成、多样性及其功能,本研究采用传统分离培养法,对杜仲叶附生及内生真菌群落组成及多样性进行分析,基于FUNGuild数据库对其进行功能注释,并对其产IAA能力进行评估。本试验共分离获得真菌2门42属226株。其中,从野生杜仲叶中分离获得154株,主要包括青霉属Penicillium(25.32%)等19个优势属;从人工栽培杜仲叶中分离获得72株,主要包括曲霉属Aspergillus(16.67%)等19个优势属。功能注释结果显示:除未定义功能类群,真菌类群主要涉及动物病原菌群(12.79%)、植物病原菌群-木腐菌群(13.95%)和内生菌群-附生菌群-植物病菌群(24.42%)。结果表明人工栽培种和野生种的真菌物种组成差别较大,且共有菌株比例较低。本研究还分离到多个产吲哚-3-乙酸(IAA)菌株。研究结果将为后续精准调控杜仲叶微生物组以增强植物健康、促进植物生长以及功能菌株的开发利用。

As a unique medicinal plant in China,Eucommia ulmoides is widely distributed in Guizhou province.The phyllosphere microorganisms are of great significance for its growth.In order to reveal the community composition,diversity,and function of phyllosphere fungi in artificially cultivated and wild Eucommia ulmoides,and to screen strains with IAA-production activity,based on the traditional cultivation methods,the composition and diversity of endophytic fungal communities in Eucommia ulmoides phyllosphere was analyzed,and their function groups were annotated based on the FUNGuild database.As a result,a total of 226 fungal strains were isolated from E.ulmoides phyllosphere belonged to 2 phyla and 42 genera.Among them,154 strains were from wild leaves,mainly including 19 dominant genera such as Penicillium(25.32%),and 72 strains were from artificially cultivated leaves,mainly including 19 dominant genera such as Aspergillus(16.67%).The functional annotation results showed that,except for undefined functional groups,fungal groups mainly involve Animal Pathogen(12.79%),Plant Pathogen Wood Saprotroph(13.95%)and Endophyte Epiphyte Plant Pathogen(24.42%).The fungal species composition of cultivated and wild Eucommia ulmoides was quite different.At the same time,this study also screened several bacterial strains producing IAA.This research will provide valuable microbial resources for the subsequent artificial regulation of E.ulmoides phyllosphere microbiota to enhance plant health,promote plant growth,and develop functional strains.