鱼酱排毒合剂对急性鼻窦炎大鼠NF-κB通路的影响

Effect of Yujiang Paidu Decoction on NF-κB Signaling Pathway of Acute Rhinosinusitis Rats

目的研究鱼酱排毒合剂对急性鼻窦炎大鼠核转录因子-κB(NF-κB)通路相关蛋白表达的影响。方法采用单侧鼻腔接种金黄色葡萄球菌建立大鼠急性鼻窦炎模型,根据急性鼻窦炎评分表评估造模结果。将成模大鼠分为正常组、模型组、鱼酱组(予鱼酱排毒合剂)和阿莫西林组(予阿莫西林克拉维酸分散片),各组给予相应药物治疗7 d。治疗结束后测定各组大鼠鼻腔分泌物的pH值;HE染色观察鼻黏膜组织病理学改变;ELISA检测鼻腔灌洗液及鼻黏膜上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)水平;Western blotting检测NF-κB信号通路相关蛋白表达水平。结果急性鼻窦炎大鼠炎症模型成功建立。与模型组相比,鱼酱组和阿莫西林组大鼠鼻腔分泌p H值显著降低(P<0.01),鼻黏膜病理损伤明显改善,鼻腔灌洗液和鼻黏膜组织上清液中TNF-α、IL-1β、IL-6水平明显降低(P<0.01),NF-κB相关蛋白表达水平显著下降(P<0.01)。结论鱼酱排毒合剂可通过调节急性鼻窦炎大鼠NF-κB相关信号蛋白的表达,从而改善大鼠急性鼻窦炎症状。

Objective:To investigate the effect of Yujiang Paidu Decoction on the expression of NF-κB path-way-associated proteins in acute rhinosinusitis rats.Methods:The rat model of acute rhinosinusitis was estab-lished through unilateral nasal inoculation with Staphylococcus aureus.The effectiveness of the model was as-sessed based on the severity scale for acute rhinosinusitis.Rats were divided into the normal,model,Yujiang Pai-du Decoction and positive drug(Amoxicillin clarithromycin)groups.The pH value of nasal secretions in each group was determined.HE staining was employed to observe the histopathological alterations of nasal mucosa.The levels of TNF-α,IL-6,and IL-1βin nasal lavage fluid and nasal mucosal supernatant were detected by ELI-SA.Rats with acute rhinosinusitis were subjected to Western blotting analysis to analyze the effect of Yujiang Pai-du Decoction on the expression levels of NF-κB signaling pathway-related proteins.Results:The acute rhinosi-nusitis rat inflammation model was successfully established.Compared with the model group,the pH value of the Yujiang Paidu Decoction and the positive drug group was significantly reduced(P<0.01),the pathological injury of nasal mucosa was significantly improved,the levels of TNF-α,IL-1β,and IL-6 in nasal lavage fluid and nasal mucosa supernatant were significantly decreased(P<0.01)and the expression level of NF-κB related protein was significantly decreased(P<0.01).Conclusion:Yujiang Paidu Decoction can ameliorate the symptoms of acute rhinosinusitis by suppressing the expression of NF-κB related signaling proteins in rats with acute rhinosi-nusitis.