七种不同产区花椒的挥发性成分研究

Comparative analysis of volatile components in Zanthoxylum bungeanum from seven different producing areas

以7个不同产地花椒作为研究对象,采用顶空固相微萃取结合气相色谱-质谱联用法对不同产地花椒中的挥发性成分进行分离鉴定差异化分析。结果表明:7种花椒中共鉴定到86种挥发性化合物,主要为烯烃类、烷烃类、醇类、酮类、酸类、酮类、酯类、醚类;含量较高的挥发性成分有桉树醇、β-蒎烯、柠檬烯、芳樟醇和乙酸芳樟酯。青花椒和红花椒中的挥发性成分差异最明显。不同种类和产地的花椒中烯醇酯类物质含量差异明显。其中柠檬烯和芳樟醇可以作为区分青红花椒的差异性物质,乙酸松油酯和乙酸芳樟酯可以作为区分花椒产地是否为西南地区的差异性物质,β-蒎烯和反式橙花叔醇可作为辅助差异性物质参与产地鉴定。

Zanthoxylum bungeanum from seven different producing areas were studied.Using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry,the volatile components of Zanthoxylum bungeanum from seven different producing areas were separated,identified and comparatively analyzed.The results showed that a total of 86 volatile compounds were identified in the seven types of Zanthoxylum bungeanum,mainly including olefins,alkanes,alcohols,ketones,acids,esters,and ethers,with eucalyptol,β-pinene,limonene,linalool,and linalyl acetate being predominant.The volatile components of green Zanthoxylum bungeanum were most notably different from red Zanthoxylum bungeanum.The volatile content,such as alcohols﹐esters,and alkenes,varied significantly among Zanthoxylum bungeanum of different types and from different producing areas.Limonene and linalool were identified as key compounds differentiating green and red Zanthoxylum bungeanum,while terpinyl acetate and linalyl acetate served as markers for Southwest China varieties of Zanthoxylum bungeanum,β-phellandrene and trans-nerolidol could participate in regional identification as auxiliary distinguishing substances.