灵长类动物心脏衰老中的单细胞转录组生物信息学研究

Single-Cell Transcriptomics Bioinformatics Study in Cardiac Aging of Primates

目的:利用单细胞转录组数据探究灵长类动物心脏衰老的潜在分子机制。方法:利用高通量测序数据库Genome Sequence Archive中查找到的和“primate cardiac aging”有关的单细胞测序原始数据集CRA002689和CRA002810,通过细胞聚类、注释和差异表达基因分析筛选调控心脏衰老可能的信号通路。结果:过滤得到220915个细胞测序数据,其中年轻组心脏细胞78546个,年老组心脏细胞142369个,聚类成15个细胞亚群,纤维原细胞(Fib)细胞亚群占比最高。上皮细胞(Epi)中差异表达基因数目最多,而抗炎巨噬细胞(M2)中差异表达基因数目最少。差异表达基因显著富集于“supramolecular fiber organization”和“actin filament-based process”等通路。结论:上皮细胞在心脏衰老过程中发生了较大的变化,其可能在心脏衰老过程中起着重要的作用,上皮细胞的“supramolecular fiber organization”和纤维原细胞“actin filament-based process”通路与衰老密切相关。

Objective:To explore the potential molecular mechanisms of cardiac aging in primates using single-cell transcriptomic data.Methods:The single-cell sequencing datasets CRA002689 and CRA002810 related to“primate cardiac aging”are retrieved from the high-throughput sequencing database Genome Sequence Archive.Cell clustering,annotation,and differential gene analysis are conducted to identify signaling pathways potentially regulating cardiac aging.Results:A total of 220915 cell sequencing data are filtered,including 78546 cells from the young heart group and 142369 from the older heart group.These are clustered into 15 cell subpopulations,with fibroblast cells(Fib)having the highest proportion.The highest number of differential genes is observed in epicardial cells(Epi),while the lowest is in anti-inflammatory macrophage cells(M2).Differentially expressed genes are significantly enriched in pathways such as“supramolecular fiber organization”and“actin filament-based process.”Conclusion:Epithelial cells undergo significant changes during cardiac aging and may play an important role in this process.The“supramolecular fiber organization”in epithelial cells and the“actin filament-based process”in fibrocytes are closely related to aging.