榆干玉蕈子实体及其提取物的免疫调节作用

Immunoregulatory Activity of Hypsizygus ulmarius Fruiting Body and Its Extracts

为研究榆干玉蕈(Hypsizygusulmarius)子实体粉末及提取物对小鼠的免疫调节作用,分别采用榆干玉蕈子实体粉末(高、低剂量分别为1.2、0.3g·kg^(-1))、石油醚提取物(高、低剂量分别为1.392、0.348mg·kg^(-1))、乙酸乙酯提取物(高、低剂量分别为3.306、0.826 mg·kg^(-1))、水提取物(高、低剂量分别为28.78、7.19 mg·kg^(-1))灌胃大黄水煎液建立的模型小鼠,测定其体质量、脾脏指数及血清中白细胞介素2(interleukin-2,IL-2)、白细胞介素6(interleukin-6,IL-6)、免疫球蛋白M(immunoglobulin M,IgM)、干扰素γ(interferon-γ,IFN-γ)、D-木糖(D-xylose)、白蛋白(albumin,ALB)、胰脂肪酶(pancrelipase,PL)含量,采用苏木素-伊红染色观察小鼠脾脏组织显微结构,采用RT-qPCR法检测维生素D结合蛋白(D binding protein,DBP)和紧密连接蛋白(occludin,OCLN)的基因表达情况,并采用Westernblotting方法检测DBP和OCLN蛋白表达情况。结果表明:与模型组相比,第14天,各给药组小鼠体质量均显著增加。粉末高剂量组脾脏指数显著增加。所有给药组D-xylose、IFN-γ、ALB、IL-2、IgM、PL含量均显著增加,IL-6含量显著降低。各给药高剂量组DBP基因相对表达量显著升高,所有低剂量组显著降低;粉末低剂量组、石油醚提取物高和低剂量组、水提物高和低剂量组的OCLN基因相对表达量均显著升高。各给药组DBP和OCLN的蛋白表达量均显著升高。榆干玉蕈子实体及各提取物均通过免疫调节发挥作用,研究结果可为榆干玉蕈进一步开发利用提供参考。

In order to study the immunomodulatory effects of Hypsizygus ulmarius fruiting body powder and its different extracts on mice,eight experimental groups were set up as follows:high-dose(1.2 g·kg^(-1))and low-dose(0.3 g·kg^(-1))fruiting body powder groups,high-dose(1.392 g·kg^(-1))and low-dose(0.348 g·kg^(-1))petroleum ether extract groups,high-dose(3.306 g·kg^(-1))and low-dose(0.826 g·kg^(-1))ethyl acetate extract groups,and high-dose(28.78 g·kg^(-1))and low-dose(7.19 g·kg-1)water extract groups.These powders and extracts were administered to mice with spleen deficiency syndrome by gavage,respectively.Then mice in different treatment groups were measured for body mass,spleen index,and serum contents of interleukin-2(IL-2),interleukin-6(IL-6),immunoglobulin M(IgM),interferon-γ(IFN-γ),D-xylose,albumin(ALB)and pancrelipase(PL).Hematoxylin-eosin staining was used to observe the microscopic structure of mouse spleen tissue.RT-qPCR was used to detect the gene expression of vitamin D-binding protein(DBP)and occludin(OCLN),and Western blotting was used to detect and semiquantify DBP and OCLN.The results showed that the body mass of mice in all treatment groups significantly increased compared with the model group at 14 d.The spleen index of the high-dose fruiting body powder group was significantly higher than that of the model group.All treatment groups showed higher contents of D-xylose,IFN-γ,ALB,IL-2,IgM and PL than the model group.In contrast,IL-6 level was significantly decreased in all treatment groups compared with the model group.Compared with the model group,the relative expression of DBP gene was significantly increased in all high-dose groups,but significantly decreased in all low-dose groups.The relative expression of OCLN gene was significantly increased in the low-dose powder group,petroleum ether extract groups,and water extract groups.The protein levels of DBP and OCLN were significantly increased in all treatment groups.These results suggested that H.ulmarius fruiting body powder and its extracts have immunomodulatory and spleen-invigorating effects,and thus provided a reference for further development and utilization of the mushroom.