变应性鼻炎患者血清miR-149-5p、Notch1 mRNA表达与Treg/Th17平衡的关系

Correlations between serum miR-149-5p and Notch1 mRNA expression and Treg/Th17 balance in patients with allergic rhinitis

目的探讨变应性鼻炎(AR)患者血清miR-149-5p、Notch1 mRNA表达与调节性T细胞(Treg)/辅助性T细胞(Th)17平衡的关系。方法选择98例AR患者(AR组)和30例健康体检志愿者(对照组),根据疾病严重程度将AR患者分为轻度组(51例)、中重度组(47例)。采用实时荧光定量聚合酶链反应检测血清miR-149-5p、Notch1 mRNA表达,采用流式法检测外周血中Treg、Th17及其细胞因子。Pearson相关性分析血清miR-149-5p、Notch1 mRNA表达与外周血中Treg、Th17及其细胞因子以及miR-149-5p与Notch1 mRNA表达的相关性。结果AR组血清miR-149-5p表达,白细胞介素(IL)-10、转化生长因子-β(1 TGF-β1)水平以及外周血Treg细胞、Treg/Th17值低于对照组(P均<0.05),血清Notch1 mRNA表达,IL-6、IL-17、IL-22、IL-23水平以及外周血Th17细胞高于对照组(P均<0.05)。中重度组血清miR-149-5p表达,IL-10、TGF-β1水平以及外周血Treg细胞、Treg/Th17值低于轻度组(P均<0.05),血清Notch1 mRNA表达,IL-6、IL-17、IL-22、IL-23水平以及外周血Th17细胞高于轻度组(P均<0.05)。AR组血清miR-149-5p表达与外周血Treg细胞、Treg/Th17值、血清IL-10、TGF-β1水平呈正相关(r分别为0.367、0.539、0.265、0.301,P均<0.05),与血清IL-6、IL-17、IL-22、IL-23水平以及外周血Th17细胞呈负相关(r分别为-0.289、-0.213、-0.336、-0.243、-0.221,P均<0.05)。Notch1 mRNA表达与外周血Treg细胞、Treg/Th17值、血清IL-10、TGF-β1水平呈负相关(r分别为-0.346、-0.508、-0.281、-0.293,P均<0.05),与血清IL-6、IL-17、IL-22、IL-23水平以及外周血Th17细胞呈正相关(r分别为0.326、0.241、0.302、0.285、0.237,P均<0.05)。血清miR-149-5p与Notch1 mRNA表达呈负相关(r=-0.550,P<0.05)。结论AR患者血清miR-149-5p表达下调,Notch1 mRNA表达上调,且与AR患者Treg/Th17失衡有关。

Objective To investigate the correlations between the expression levels of miR-149-5p and Notch1 mRNA in serum and the balance of regulatory T cells(Treg)/helper T cells(Th)17 in patients with allergic rhinitis(AR).Methods Ninety-eight AR patients(AR group)and 30 healthy volunteers(control group)were selected.According to the severity of the disease,the patients with AR were divided into the mild group(51 cases)and moderate and severe group(47 cases).Real-time fluorescence quantitative PCR was used to detect the expression of miR-149-5p and Notch1 mRNA in serum,and flow cytometry was used to detect Treg,Th17 and their cytokines in peripheral blood.Pearson correlation was used to analyze the correlations between serum miR-149-5p,Notch1 mRNA expression and peripheral blood Treg,Th17 and their cytokines,as well as the relationship between the miR-149-5p and Notch1 mRNA expression.Results Serum miR-149-5p expression,interleukin(IL)-10,transforming growth factor-β1(TGF-β1)level,peripheral blood Treg cells and Treg/Th17 ratio in the AR group were lower than those in the control group(all P<0.05),and serum Notch1 mRNA ex-pression,IL-6,IL-17,IL-22,IL-23 and Th17 cells in peripheral blood were higher than those in the control group(all P<0.05).The expression of miR-149-5p,the levels of IL-10,TGF-β1,Treg cells and Treg/Th17 ratio in peripheral blood were lower in the moderate and severe group than in the mild group(all P<0.05),and the expression of Notch1 mRNA,the levels of IL-6,IL-17,IL-22,IL-23 and Th17 cells in peripheral blood were higher than those in the mild group(all P<0.05).Serum miR-149-5p expression in the AR group was positively correlated with peripheral blood Treg cells,Treg/Th17 ratio,serum IL-10,and TGF-β1 levels(r=0.367,0.539,0.265,0.301,respectively;all P<0.05),and was nega-tively correlated with the levels of serum IL-6,IL-17,IL-22,IL-23 and peripheral blood Th17 cells(r=-0.289,-0.213,-0.336,-0.243,-0.221,respectively,all P<0.05).Notch1 mRNA expression was negatively correlated with peripheral blood Treg cells,Treg/Th17 ratio,serum IL-10,and TGF-β1 level(r=-0.346,-0.508,-0.281,-0.293,respectively;all P<0.05),and was positively correlated with serum IL-6,IL-17,IL-22,IL-23 levels and peripheral blood Th17 cells(r=0.326,0.241,0.302,0.285,0.237,respectively,all P<0.05).Serum miR-149-5p was negatively correlated with Notch1 mRNA expression(r=-0.550,P<0.05).Conclusion The expression of miR-149-5p is down-regulated and Notch1 mRNA is up-regulated in AR patients,which are related to the imbalance of Treg/Th17 in AR patients.