摘要
The H9N2 subtype of avian influenza virus(AIV)is widely prevalent in poultry and wild birds globally,and has become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly(by serving as a"donor virus")infect humans,posing a significant threat to public health.Currently,there is a lack of in-depth research on the prevalence of H9N2 viruses in Shanxi Province,central China.In this study,we isolated 14 H9N2 AIVs from October 2020 to April 2022 in Shanxi Province,and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed high transmission efficiency among chicken populations,and exhibited diverse replication abilities within these birds.These viruses could replicate efficiently in the lungs of mice,with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level,the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays,revealing significant differences in the replication and proliferation efficiency of the various H9N2 viruses at the cellular level.The antigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.
基金
Fundamental Research Program of Shanxi Province,China(202103021224156)
National Natural Science Foundation of China(32202788)
Special Research Fund of Shanxi Agricultural University for High-level Talents,China(2021XG004)
Science and Technology Innovation Program of Shanxi Agricultural University,China(2021BQ78)
special fund for Science and Technology Innovation Teams of Shanxi Province,China(202304051001041)
?Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021005,SXBYKY2021063,SXBYKY2022014)
the Fund for Shanxi“1331 Project”,China(20211331-13)
earmarked fund for Modern Agro-industry Technology Research System of Shanxi Province,China.
