PTEN、CA125、sVEGFR1、NGAL在子宫内膜癌患者血清中的表达及与病理特征的关系

Serum levels of PTEN,CA125,sVEGFR1,and NGAL in patients with endometrial carcinoma and their relationship with pathological features

目的 探讨第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)、糖类抗原125(CA125)、可溶性血管内皮生长因子受体-1(sVEGFR1)、中性粒细胞明胶酶相关脂质载运蛋白(NGAL)在子宫内膜癌(EC)患者血清中的表达及与病理特征的关系。方法 选取2019年1月至2021年6月于在邯郸市第一医院行全子宫切除术并经病理诊断的120例EC患者为研究组,选择同期80例良性病变子宫内膜患者为对照组,用酶联免疫吸附法检测并比较2组患者血清PTEN、CA125、sVEGFR1、NGAL水平,收集2组临床病理资料,分析血清PTEN、CA125、sVEGFR1、NGAL与研究组患者病理特征的关系。结果 研究组血清CA125、NGAL水平高于对照组,血清PTEN、sVEGFR1水平低于对照组(P<0.05)。分化程度越低血清CA125、NGAL水平越高,血清PTEN、sVEGFR1水平越低(P<0.05);临床分期Ⅲ~Ⅳ期患者血清PTEN高于Ⅰ~Ⅱ期(P<0.05);临床分期Ⅲ~Ⅳ期、有淋巴结转移、浸润深度≥50%患者血清CA125、NGAL水平升高,血清sVEGFR1水平降低(P<0.05)。血清PTEN与临床分期呈负相关,与分化程度呈正相关(P<0.05);血清CA125、NGAL与临床分期、淋巴结转移、浸润深度呈正相关,与分化程度呈负相关(P<0.05);血清sVEGFR1与临床分期、淋巴结转移、浸润深度呈负相关,与分化程度呈正相关(P<0.05)。结论 CA125、NGAL在EC患者血清中呈高表达,PTEN、sVEGFR1呈低表达,均与EC患者病理特征有一定相关性,可作为EC早期诊断与疾病进展的潜在生物学标志物。

Objective To measure serum levels of phosphatase and tensin homologous gene(PTEN),carbohydrate antigen 125(CA125),soluble vascular endothelial growth factor receptor-1(sVEGFR1),and neutrophil gelatinase-associated lipocalin(NGAL) on chromosome 10 deletion of patients with endometrial carcinoma(EC),and to explore their relationships with pathological features.Methods One hundred and twenty EC patients who underwent total hysterectomy with pathological diagnosis in the First Hospital of Handan from January 2019 to June 2021 were selected as the study group,and 80 patients with benign lesions of the endometrium in the same period were selected as the control group.Serum levels of PTEN,CA125,sVEGFR1,and NGAL were detected and compared between the two groups of patients with enzyme-linked immunosorbent assay(ELISA).Clinicopathological data were collected to analyze the relationship with serum PTEN,CA125,sVEGFR1,and NGAL.Results Serum CA125 and NGAL levels in the study group were significantly higher than those of the control group,and serum PTEN and sVEGFR1 levels were significantly lower than those of the control group(P<0.05).Serum CA125 and NGAL levels were significantly higher,while serum PTEN and sVEGFR1 levels were significantly lower in patients with lower degree of differentiation(P<0.05).Serum PTEN was significantly higher in patients with clinical stage Ⅲ-Ⅳ than those with stage Ⅰ-Ⅱ(P<0.05).Patients with clinical stage Ⅲ-Ⅳ,lymph node metastasis,and infiltration depth ≥50% had elevated serum CA125 and NGAL levels and decreased serum sVEGFR1 levels(P<0.05).Serum PTEN was negatively correlated with clinical stage and positively correlated with the degree of differentiation(P<0.05).Serum CA125 and NGAL were positively correlated with clinical stage,lymph node metastasis,and infiltration depth,and negatively correlated with the degree of differentiation(P<0.05).Serum sVEGFR1 was negatively correlated with clinical stage,lymph node metastasis,and infiltration depth,and positively correlated with the degree of differentiation(P<0.05).Conclusion Serum levels of CA125,and NGAL increase and serum levels of PTEN and sVEGFR1 decrease in EC patients,which are all correlated with the pathologic features of EC,and can be used as potential biomarkers for the early diagnosis and disease progression of EC.