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RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

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摘要 The chloride channel 7 gene(CLC 7)of the Hong Kong oyster Crassostrea hongkongensis was cloned and named ChCLC 7.The cDNA was 2572 bp in length,with a 5′non-coding region containing 25 bp,a 3′non-coding region containing 327 bp,and an open reading frame of 2298 bp.ChCLC 7 has 96.8%and 92.1%homology with CLC 7 of Crassostrea gigas and Crassostrea virginica,respectively,and it was clustered with CLC 7 of C.gigas and C.virginica.QRT-PCR showed that ChCLC 7 was expressed in all eight tissues,with the highest in adductor muscle and second in gill.The ChCLC 7 expression pattern in gill was altered significantly under high salinity stress with an overall upward and then downward trend.After RNA interference,the expression of ChCLC 7 and survival rate of oyster under high salinity stress was reduced significantly,and so did the concentration of hemolymph chloride ion in 48-96 h after RNA interference.We believed that ChCLC 7 could play an important role in osmoregulation of C.hongkongensis by regulating Cl^(-)transport.This study provided data for the analysis of molecular mechanism against oyster salinity stress.
出处 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第4期1261-1271,共11页 海洋湖沼学报(英文)
基金 Supported by the Natural Science Foundation of Guangxi Province(Nos.2023 GXNSFAA 026503,2018 GXNSFBA281201) the Guangxi Key Research and Development Program(No.GuikeAB21196030) the Marine Science Guangxi First-Class Subject,Beibu Gulf University(No.DRC002) the Scientific Research and Technology Development Plan Project of Qinzhou(Nos.202014842,20223637) the Science and Technology Major Project of Guangxi Province(No.AA17204095-10) the Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation,Beibu Gulf University(Nos.2020ZB09,2020ZB04)。
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