梅毒血清固定患者中NOD样受体蛋白3和Toll样受体4表达与Th1/Th2相关细胞因子的相关性研究

The correlation between the expression of NOD-like receptor protein 3 and Toll-like receptor 4 and T helper cell 1/T helper cell 2 related cytokines in patients with syphilis serum fixation

目的探究梅毒血清固定患者中NOD样受体蛋白3(NLRP3)、Toll样受体4(TLR4)表达与辅助性T细胞1/辅助性T细胞2(Th1/Th2)相关细胞因子的相关性。方法选取2021年2月至2022年4月川北医学院附属三台医院收治的197例梅毒患者作为研究对象。将进行驱梅治疗后血清转阴者纳入转阴组(n=88),未进行驱梅治疗者纳入梅毒组(n=45),接受驱梅治疗后血清固定者纳入固定组(n=64)。另选取同期进行体检的健康人作为对照组(n=53)。采用实时荧光定量聚合酶链反应检测外周血单个核细胞(PBMCs)中NLRP3、TLR4 mRNA相对表达水平;采用酶联免疫吸附试验法检测Th1/Th2相关细胞因子的表达水平;采用Pearson法分析TLR4、NLRP3 mRNA与Th1/Th2相关细胞因子的相关性。结果各组PBMCs中TLR4、NLRP3 mRNA表达水平比较,梅毒组>转阴组>对照组>固定组,差异具有统计学意义(P<0.05);各组白介素(IL)-2、γ干扰素(IFN-γ)水平比较,对照组>转阴组>梅毒组>固定组,差异具有统计学意义(P<0.05);各组IL-1β、IL-4、IL-10及IL-18水平比较,对照组<转阴组<梅毒组<固定组,差异具有统计学意义(P<0.05);梅毒血清固定患者TLR4、NLRP3 mRNA表达与IFN-γ、IL-2呈正相关,与IL-1β、IL-4、IL-10、IL-18呈负相关(P<0.05)。结论梅毒血清固定患者NLRP3、TLR4 mRNA表达水平显著降低,且与Th1/Th2相关细胞因子密切相关。

Objective To investigate the correlation between NOD-like receptor protein 3(NLRP3)and Toll-like receptor 4(TLR4)expression and T helper cell 1/T helper cell 2(Th1/Th2)related cytokines in patients with syphilis serum fixation.Methods A total of 197 syphilis patients admitted to Santai Hospital,North Sichuan Medical College from February 2021 to April 2022 were selected as the study objects.Patients with serum negative conversion after anti syphilis treatment were selected as the negative conversion group(n=88),patients who did not receive anti syphilis treatment were collected as the syphilis group(n=45),patients with serum fixation after anti syphilis treatment were selected as the fixation group(n=64).Healthy people who underwent physical examination during the same period were selected as the control group(n=53).The relative expression levels of NLRP3,TLR4 mRNA in peripheral blood mononuclear cells(PBMCs)were detected by real-time fluorescence quantitative polymerase chain reaction.The expression level of Th1/Th2 related cytokines was detected by enzyme-linked immunosorbent assay;Pearson method was applied to analyze the correlation between NLRP3,TLR4 mRNA and Th1/Th2 related cytokines.Results The expression levels of TLR4,NLRP3 mRNA in PBMCs of all groups were compared,syphilis group>negative group>control group>fixed group,and the differences were statistically significant(P<0.05).The levels of interleukin(IL)-2 and interferon(IFN)-γin all groups were compared,control group>negative group>syphilis group>fixed group,with statistical significance(P<0.05).The levels of IL-1β,IL-4,IL-10 and IL-18 in all groups were compared,control group<negative group<syphilis group<fixed group,with statistical significances(P<0.05).The expression of TLR4,NLRP3 mRNA were positively correlated with IFN-γand IL-2,and negatively correlated with IL-1β,IL-4,IL-10 and IL-18 in patients with syphilis serum fixation(P<0.05).Conclusions The expression levels of NLRP3,TLR4 mRNA were significantly decreased in patients with syphilis serum fixation,and were closely related to Th1/Th2 related cytokines.