摘要
Grain size is a key factor influencing grain weight in rice.In this study,a chromosome segment substitution line(CSSL9-17)was identified,that exhibits a significant reduction in both grain size and weight compared to its donor parent 93-11.Further investigation identified two quantitative trait loci(QTL)on chromosome 11,designated qGW11a and qGW11b,which contribute to 1000-grain weight with an additive effect.LOC_Os11g05690,encoding the amino acid permease OsCAT8,is the target gene of qGW11a.Overexpression of OsCAT8 resulted in decreased grain weight,while OsCAT8 knockout mutants exhibited increased grain weight.The 287-bp located within the OsCAT8 promoter region of 93-11 negatively regulates its activity,which is subsequently correlated with an increase in grain size and weight.These results suggest that OsCAT8 functions as a negative regulator of grain size and grain weight in rice.
基金
supported by grants from the National Natural Science Foundation of China(32325038)
the Postdoctoral Fellowship Program of CPSF(GZB20230499)
the Sichuan Science and Technology Program(24NSFSC4494)
the Open Project Program(SKL-ZY202212)of State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China.We thank the High-Performance Computing Platform of Sichuan Agricultural University for its support for the analysis of substitution segments in CSSL9-17.
