卷丹多糖的结构表征及其对PM_(2.5)暴露致小鼠急性肺损伤的影响

Structural characterization of polysaccharide from Lilii Lancifolii Bulbus and its effect on acute lung injury in mice induced by PM_(2.5)exposure

对卷丹多糖(Lilii Lancifolii Bulbus polysaccharide,LP)的结构进行表征,并考察卷丹水提液(Lilii Lancifolii Bulbus water extract,LW)及其多糖部位对PM_(2.5)暴露致小鼠急性肺损伤的保护作用。采用水提醇沉法得到LP并利用苯酚-硫酸法测定其总糖含量,利用纤维素层析法对其进行分离纯化,采用凝胶渗透色谱以及1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)柱前衍生化法对LP结构进行分析,并考察了LW和LP对PM_(2.5)暴露致小鼠急性肺损伤的影响。结果测得LP的总糖含量为(70.98±2.36)%,分离纯化得到LP主要成分LP-1的相对分子量为5906 Da,主要由甘露糖和葡萄糖组成,摩尔比为1.00∶2.01。与空白组相比较,模型组小鼠体重增势缓慢(P<0.01),肺组织损伤严重,肺指数以及肺组织中的炎症因子平增加(P<0.01)。与模型组相比较,阳性药(地塞米松)组以及LW和LP组小鼠体重显著增长(P<0.01),肺组织结构均相对完整,肺指数、肺组织病理评分、炎症因子水平显著降低(P<0.05)。并且,相同给药剂量下,LW与LP组之间的疗效基本一致,表明卷丹多糖可能是卷丹有效改善肺损伤小鼠的肺部组织形态,减少肺部炎症因子表达,发挥PM_(2.5)暴露致急性肺损伤疗效的重要药效物质基础。

This study aims to characterize the structure of Lilii Lancifolii Bulbus polysaccharide(LP)and investigate the protective effect of the Lilii Lancifolii Bulbus water extract(LW)and LP on acute lung injury induced by PM_(2.5)exposure in mice.LP was obtained by water-extraction and alcohol-precipitation method.The LP was separated and purified by cellulose chromatography,and its structure was analyzed by gel permeation chromatography and 1-phenyl-3-methyl-5-pyrazolinone(PMP)pre-column derivatization.More to the point,the effects of LW and LP on acute lung injury induced by PM_(2.5)exposure in mice were investigated.The results show that total sugar content of LP was(70.98±2.36)%by phenol-sulfuric acid method,and the relative molecular weight of LP-1 was 5906 Da,consisting of mannose and glucose in a molar ratio of 1.00∶2.01.In the pharmacodynamic study,compared with the control group,the weight increase of the model group was slow(P<0.01),the lung tissue damage was serious,the lung index and the level of inflammatory factors in the lung tissue were increased(P<0.01).Compared with the model group,the body weight of the mice in the positive drug(dexamethasone)group and the LW and LP groups increased significantly(P<0.01),the lung tissue structure was relatively intact,and the lung index,lung tissue pathological score and inflammatory factor levels were significantly reduced(P<0.05).In addition,at the same dose,the efficacy of LW and LP group were basically the same,indicating that the polysaccharide may be an important medicinal substances basis for Lilii Lancifolii Bulbus to effectively improve the lung tissue morphology of mice with lung injury,reduce the expression of inflammatory factors in lung,and play an important role in the therapeutic effect of acute lung injury induced by PM_(2.5)exposure.