养心草提取物抗肺癌活性部位筛选

Screening of Active Fractions from Yangxincao(Aizoon Stonecrop Herb)for Anti-lung Cancer

目的筛选养心草抗肺癌活性部位。方法运用系统溶剂法制备养心草各提取部位;体外培养肝癌A549细胞,采用MTT法检测细胞株半数抑制浓度(IC50),以IC50作为评价指标,初步筛选出养心草抗肿瘤的有效部位。同时,建立肺癌LLC细胞移植瘤小鼠模型,通过观察肿瘤体积、小鼠体质量、脏器指数及瘤体质量的变化,对养心草的抗肿瘤效果进行评价养心草石油醚部位及乙酸乙酯部位的体内抗肺癌作用。结果MTT实验结果表明养心草不同提取部位中石油醚部位和乙酸乙酯部位对肺癌A549细胞的增殖显示较强的抑制作用;体内小鼠肺癌LLC细胞移植瘤实验表明石油醚部位和乙酸乙酯部位可明显抑制小鼠肿瘤的生长(P<0.05),且具有剂量依赖性,并对鼠体质量及脏器指数未产生显著影响(P>0.05)。结论养心草的石油醚部位和乙酸乙酯部位是其抗肺癌的有效部位。

Objective To screen the active parts of Yangxincao(Aizoon Stonecrop Herb)for antilung cancer in vivo.Methods The various extract parts were prepared using the systematic solvent method.The half-inhibitory concentration(IC50)of A549 lung cancer cells was detected using the MTT method,and the IC50 was used as the evaluation indicator to preliminarily screen out the effective parts.Meanwhile,a mouse model of LLC lung cancer cell transplantation was established to observe changes in tumor volume,mouse body weight,organ indices,and tumor weight to evaluate the in vivo anti-lung cancer effects of the petroleum ether and ethyl acetate parts of Yangxincao(Aizoon Stonecrop Herb).Results The results showed that the petroleum ether and ethyl acetate parts of Yangxincao(Aizoon Stonecrop Herb)had a strong inhibitory effect on the proliferation of A549 lung cancer cells and could significantly inhibit the growth of mouse tumors(P<0.05),with a dose-dependent effect.There was no significant effect on mouse body weight and organ index(P>0.05).Conclusion The petroleum ether and ethyl acetate parts of Yangxincao(Aizoon Stonecrop Herb)are the effective fractions against lung cancer.