液相色谱质谱联用分离鉴定酸性植物激素的初级教学实验

Elementary teaching experiment on the separation and identification of acidic plant hormones by LC-MS

液相色谱质谱联用仪(LC-MS)是目前检测脱落酸、吲哚乙酸等植物激素的重要技术手段之一。为了巩固学生对LC-MS基本概念与原理的掌握,提高学生的实践能力,培养学生实验素养,针对目前国内LC-MS实验教学存在的主要问题,本文提供了从易至难的5个基础教学实验,可使学生了解LC-MS样品前处理的基本过程,认识实际样品的复杂性和痕量植物激素检测的瓶颈问题,初步了解实际样品中ABA含量的计算方法。连续3个学期在研究生一年级仪器分析课的实践表明,这5个教学实验的学习实践活动能培养学生使用现代大型分析仪器的能力,提升学生学习仪器分析技术的兴趣。

Liquid chromatography-mass spectrometry(LC-MS)is currently one of the important technical means for the detection of plant hormones such as abscisic acid(ABA)and indole-3-acetic acid.In order to consolidate students’grasp of the basic concepts and principles of LC-MS,improve their practical abilities and cultivate their experimental literacy,this paper provides 5 basic teaching experiments,ranging from easy to difficult,to address the main problems in current domestic LC-MS experimental teaching.These experiments can help students understand the basic process of LC-MS sample pretreatment,recognize the complexity of real samples and the bottleneck issues in the determination of trace plant hormones and learn preliminarily the method for the calculation of ABA content in real samples.After consecutive practice for three semesters in the Instrument Analysis course for the first year graduate students,these five teaching experiments have shown their teaching efficiency to cultivate the ability for students to use modern large-scale analytical instruments and to enhance their interest to learn instrument analysis technology.