姜黄素对人视网膜色素上皮细胞中基质金属蛋白酶、纤维粘连蛋白1表达的影响观察

Effect of curcumin on expression of matrix metalloproteinase and fibronectin in human retinal pigment epithelium

目的观察姜黄素对人视网膜色素上皮(RPE)细胞中基质金属蛋白酶(MMP)、纤维粘连蛋白1(FN1)表达的影响。方法将人RPE细胞分成2组,姜黄素组加入0.5×10^(-5)mol/L的姜黄素,对照组不加姜黄素,两组细胞继续孵育48 h后,采用实时荧光定量PCR法检测细胞中MMP1、MMP2、MMP3、MMP9、FN1 mRNA,采用Western blotting法检测细胞中MMP1、MMP2、MMP3、MMP9、FN1蛋白。结果姜黄素组细胞中MMP1、MMP2、MMP3、MMP9、FN1 mRNA相对表达量分别为0.41±0.14、5.02±0.22、1.09±0.21、0.47±0.28、2.50±0.21,对照组分别为5.10±0.38、6.43±0.15、0.27±0.19、1.82±0.12、6.83±0.18,两组相比,P均<0.05。姜黄素组细胞中MMP1、MMP2、MMP3、MMP9、FN1蛋白相对表达量分别为6155.33±124.01、6208.00±157.33、8126.33±235.78、3541.67±130.16、3684.33±90.22,对照组分别为16207.33±210.19、8053.33±61.98、2543.67±122.20、9477.33±108.68、8218.00±239.86,两组相比,P均<0.05。结论姜黄素可上调人RPE细胞中MMP1、MMP2、MMP9、FN1的表达,下调MMP3的表达。

Objective To observe the effect of curcumin on the expression of matrix metalloproteinase(MMP)and fibronectin 1(FN1)in human retinal pigment epithelium(RPE).Methods Human RPE cells were divided into two groups:the curcumin group and control group;cells in the curcumin group were added with 0.5×10^(-5) mol/L curcumin,and cells in the control group were not treated with curcumin.After cells of the two groups were continuously incubated for 48 h,real-time fluorescence quantitative PCR was used to detect the matrix metalloproteinase(MMP)1,MMP2,MMP3,MMP9,and FN1 mRNAs,and Western blotting was used to detect the MMP1,MMP2,MMP3,MMP9,FN1 proteins in cells.Results The relative expression levels of MMP1,MMP2,MMP3,MMP9,and FN1 mRNAs in cells in the curcumin group were 0.41±0.14,5.020.22,1.090.21,0.470.28,and 2.500.21,and those in the control group were 5.100.38,6.430.15,0.270.19,1.820.12,and 6.830.18,respectively,with statistically significant differences be‑tween these two groups(all P<0.05).The relative expression levels of MMP1,MMP2,MMP3,MMP9,and FN1 pro‑teins were 6155.33124.01,6208.00157.33,8126.33235.78,3541.67130.16,3684.3390.22,respectively,in the curcumin group,versus 16207.33210.19,8053.3361.98,2543.67122.20,9477.33108.68,and 8218.00239.86,respectively,in the control group,with statistically significant differences between these two groups(all P<0.05).Conclusion Curcumin up-regulates the expression of MMP1,MMP2,MMP9,FN1 and down-regulates the expression of MMP3 in human RPE cells.