三丁酸甘油酯通过调节AMPK/mTOR通路改善鸡白痢沙门氏菌诱导的肝脏炎症

Tributyrin Improves Liver Inflammation Induced by Salmonella Pullorum by Regulating AMPK/mTOR Pathway

为探讨三丁酸甘油酯(TB)对鸡白痢沙门氏菌(SP)诱导的肝脏炎症调控机制,试验将128只1日龄健康、体重相近的海南文昌鸡随机分为对照组(Con组)、SP模型组(Mod组)、SP+恩诺沙星抗生素组(Ant组)、SP+三丁酸甘油酯组(Tre组),每组4个重复,每个重复8只。试验比较了各组雏鸡的生长性能,并通过血液生化检测、肝脏HE染色、荧光实时定量PCR和免疫组化等方法探究TB对SP所致肝脏炎症的影响及其调控机制。结果显示:①Ant组和Tre组平均日增重(ADG)、平均日采食量(ADFI)、料重比(F/G)和存活率均显著高于Mod组(P<0.05);②Ant组和Tre组γ-谷氨酰转移酶(GGT)、谷草转氨酶(AST)、谷丙转氨酶(ALT)活性和总胆红素(TBIL)含量显著低于Mod组(P<0.05),白蛋白(ALB)水平显著高于Mod组(P<0.05);③与Con组相比,Ant组肝脏细胞形态完整,有少量炎性细胞,Tre组肝脏细胞形态较完整,伴有少量炎性细胞浸润;④Ant组和Tre组促炎因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(INF-γ)、诱导型一氧化氮合酶(iNOS)mRNA表达水平较Mod组显著降低(P<0.05),而白细胞介素-10(IL-10)mRNA表达水平较Mod组显著升高(P<0.05);⑤恩诺沙星和TB可通过AMPK/mTOR和NF-κB通路减轻SP感染对雏鸡肝脏损伤的影响。研究表明,饲粮添加TB可显著提高SP感染雏鸡的生长性能,降低部分血液生化指标,可能通过AMPK/mTOR通路减轻SP所致的肝脏损伤,并减少促炎因子的表达以减缓肝脏炎症。

In order to explore the regulatory mechanism of triglyceride(TB)on Salmonella Pullorum(SP)induced liver in⁃flammation,1281-day-age healthy Hainan Wenchang chickens were randomly divided into the control group(Con group),SP model group(Mod group),SP+enrofloxacin antibiotic group(Ant group)and SP+tributyrate treatment group(Tre group)with 4 rep⁃licates per group and 8 chickens per replicate.The study compared the growth performance of chicks and explored the effect of tri⁃butylglycerol(TB)on liver inflammation caused by SP and its regulatory mechanism by blood biochemical testing,liver HE stain⁃ing,fluorescence real-time quantitative PCR and immunohistochemistry.The results showed that:①The average daily gain(ADG),average daily feed intake(ADFI),feed conversion ratio(F/G)and survival rate of Ant and Tre groups were significantly higher than those of Mod group(P<0.05);②The activity ofγ-glutamyltransferase(GGT),aspartate aminotransferase(AST),alanine aminotransferase(ALT)and total bilirubin(TBIL)content in Ant and Tre groups were significantly lower than those in Mod group(P<0.05),and albumin(ALB)levels were significantly higher than those in Mod group(P<0.05);③Compared with Con group,the liver cells in Ant group were intact with a small amount of inflammatory cells,and the liver cells in Tre group were more intact with a small amount of inflammatory cell infiltration;④The mRNA expression levels of pro-inflammatory factor interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-12(IL-12),tumour necrosis factor-α(TNF-α),interferon-γ(INF-γ),inducible nitric oxide synthase(iNOS)in Ant and Tre groups were significantly decreased compared with those in Mod group(P<0.05),while the mRNA expression level of interleukin-10(IL-10)was significantly increased compared with that in Mod group(P<0.05);⑤Enrofloxacin and TB could be detected through AMPK/mTOR and NF-κB pathway to reduce the impact of SP infection on liver damage in chicks.The results indicated that dietary TB could significantly improve the growth performance of SP-infected chicks and reduce some blood biochemical indices,which could alleviate SP-induced liver damage in chicks through the AMPK/mTOR pathway and reduce the expression of pro-inflammatory factors to slow down liver inflammation.