血管内皮生长因子A与小泛素相关修饰物特异性蛋白酶5在前列腺癌中的表达及临床意义

Expression and clinical significance of vascular endothelial growth factor A and small ubiquitin associated modifier specific proteinase 5 in prostate cancer

目的:探讨血管内皮生长因子A(VEGFA)与小泛素相关修饰物特异性蛋白酶5(SENP5)在前列腺癌中的表达情况及临床意义。方法:使用GEPIA数据库,对VEGFA、SENP5基因的相关性及与前列腺癌病人的生存期进行分析;采用qRT-PCR、Western blotting从转录、蛋白质翻译水平检测肿瘤细胞中VEGFA、SENP5的表达情况;收集前列腺癌病人组织标本和临床资料,采用免疫组织化学法检测前列腺癌组织中VEGFA、SENP5的蛋白表达,分析其表达情况与肿瘤病人临床病理参数间的关系;使用STRING网站进行VEGFA、SENP5蛋白质互作网络图构建。结果:VEGFA与前列腺癌病人总生存时间有关(P<0.05),SENP5与病人总生存时间、无病进展生存时间均相关(P<0.05);与正常细胞相比,VEGFA与SENP5在前列腺癌细胞中mRNA、蛋白质均高表达(P<0.05);VEGFA与SENP5蛋白在肿瘤病理组织中可见淡黄色或棕黄色染色,VEGFA主要于细胞质中表达,SENP5主要于细胞核中表达;VEGFA强阳性表达与临床分期、有无远处转移相关(P<0.05),SENP5强阳性表达与Gleason评分、有无远处转移相关(P<0.05);VEGFA与SENP5间显著相关(P<0.01);SENP5可能通过SUMO1-HSP90AA1轴与VEGFA相互作用。结论:VEGFA、SENP5在前列腺癌中高表达,两者显著相关,且与肿瘤临床病理参数有关,可能是前列腺癌病人治疗的潜在靶点。

Objective:To investigate the expression levels of vascular endothelial growth factor A(VEGFA)and small ubiquitin associated modifier specific proteinase 5(SENP5)in prostate cancer,and their clinical significance.Methods:The GEPIA database was used to analyze the correlation between VEGFA,SENP5 genes and survival of prostate cancer patients.The qRT-PCR and Western blotting were used to detect the mRNA and protein expression levels of VEGFA and SENP5 in cancer cells.The tissue samples and clinical data of prostate cancer patients were collected,and the protein expressions of VEGFA and SENP5 in prostate cancer tissues were detected by immunohistochemical method,and the relationship between their expression levels and clinicopathological parameters of cancer patients were analyzed.The VEGFA and SENP5 protein interaction network map was constructed by using STRING website.Results:The VEGFA was correlated with the overall survival of prostate cancer patients(P<0.05),and the SENP5 was correlated with overall survival and progression-free survival of patients(P<0.05).Compared with the normal cells,the mRNA and protein of VEGFA and SENP5 were highly expressed in prostate cancer cells(P<0.05).Light yellow or brown yellow staining of VEGFA and SENP5 protein could be seen in tumor pathological tissues.VEGFA was mainly expressed in cytoplasm,while SENP5 was mainly expressed in nucleus.The strong positive expression of VEGFA was correlated with the clinical stage and presence or absence of distant metastasis(P<0.05),and the strong positive expression of SENP5 was correlated with the Gleason score and presence or absence of distant metastasis(P<0.05).There was significant correlation between VEGFA and SENP5(P<0.01).SENP5 might interact with VEGFA via the SUMO1-HSP90AA1 axis.Conclusions:VEGFA and SENP5 are highly expressed in prostate cancer,and related to tumor clinicopathological parameters,which may be potential therapeutic targets for prostate cancer patients.