基于超高效液相色谱-串联质谱技术分析‘福红’李冷藏期间初生代谢物动态变化规律

Analysis of Dynamic Changes in Primary Metabolites of ‘Fuhong’ Plum Fruits during Refrigeration byUltra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

为探究‘福红’李冷藏过程中初生代谢物组成及动态变化规律,采用超高效液相色谱-串联质谱技术分析3个冷藏阶段果肉初生代谢谱。将‘福红’李置于4℃贮藏,分别于0、30 d和60 d采集果肉样本,采用超高效液相色谱-串联质谱技术进行定性分析,采用正交偏最小二乘判别分析等方法筛选差异代谢物,并对差异代谢物进行通路富集分析。结果显示,从‘福红’李果肉中共检测出糖类、有机酸、氨基酸及其衍生物、脂质和核苷酸等573种代谢物。不同冷藏期间‘福红’李果肉代谢物差异显著,其中,30 d vs 0 d存在95种差异代谢物,60 d vs 30 d存在99种差异代谢物,60 d vs 0 d存在173种差异代谢物。通路富集分析显示,‘福红’李冷藏期间亚油酸代谢、不饱和脂肪酸的生物合成、丙酸代谢、硫代谢、嘌呤代谢、核苷酸代谢及半胱氨酸和蛋氨酸代谢等主要代谢通路差异显著。本研究揭示了不同冷藏期间‘福红’李果肉初生代谢物变化规律,可为‘福红’李果实品质评价与采后贮藏研究提供重要理论参考。

To understand the composition and dynamic changes of primary metabolites in‘Fuhong’plum fruits during refrigeration,the primary metabolite profile of‘Fuhong’plum fruits stored at 4℃for 0,30,and 60 days was analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Differential metabolites were selected by orthogonal partial least squares-discriminant analysis(OPLS-DA)and subjected to pathway enrichment analysis.The results showed that a total of 573 metabolites were identified,including sugars,organic acids,amino acids and their derivatives,lipids,and nucleotides.There was a significant difference in primary metabolite profile between the three storage times,and 95 differential metabolites at day 30 vs.0,99 differential metabolites at day 60 vs.30,and 173 differential metabolites at day 60 vs.0 were identified.The pathway enrichment analysis showed that the major metabolic pathways with a significant difference included linoleic acid metabolism,unsaturated fatty acid biosynthesis,propionic acid metabolism,sulfur metabolism,purine metabolism,nucleotide metabolism,cysteine and methionine metabolism.This study provides a theoretical basis for the quality evaluation and postharvest preservation of‘Fuhong’plum fruits.