饮品习惯与男性不育症因果关系的孟德尔随机化研究

Causal relationship between beverage habits and male infertility:a Mendelian randomization study

目的通过孟德尔随机化分析方法探究饮茶、喝咖啡与男性不育症之间的因果关系。方法基于公开发表的欧洲地区人群饮茶、喝咖啡与男性不育症基因组关联研究(GWAS)汇总数据,采用与饮茶、喝咖啡相关的遗传位点作为工具变量,使用逆方差加权法(IVW)作为主要分析方法进行两样本孟德尔随机化(MR)分析;采用异质性检验、多效性检验和留一法进行敏感性分析,评估分析结果的稳健性。结果IVW结果显示,饮茶、喝咖啡与男性不育症无相关性(饮茶:OR=0.75,P=0.71>0.05;喝咖啡:OR=1.46,P=0.51>0.05)。敏感性分析结果稳健,单核苷酸多态性(SNP)不存在水平多效性和异质性。结论饮茶、喝咖啡不会增加男性不育症的发病风险。

Objective To investigate the causal relationship between tea intake or coffee intake and male infertility by Mendelian randomization(MR)study.Methods Based on the published genome-wide association study(GWAS)of tea intake or coffee intake and male infertility in the Europe population,genetic loci associated with tea intake or coffee intake was used as instrumental variables,and inverse variance weighting(IVW)was used as the main analysis method to conduct a two-sample MR analysis.Heterogeneity test,pleiotropy test and leave-one-out method were used for sensitivity analysis to evaluate the robustness of the analysis results.Results The IVW method showed no correlation between tea intake or coffee intake and male infertility(tea intake:OR=0.75,P=0.71>0.05;coffee intake:OR=1.46,P=0.51>0.05).Sensitivity analysis results were robust,with no evidence of horizontal pleiotropy or heterogeneity among single nucleotide polymorphism(SNP).Conclusion Tea intake or coffee intake does not increase the risk of male infertility.