鹿鞭龟甲药对滋阴补肾缓解体力疲劳作用机制

Mechanism of deer penis and tortoise shellmedicine in nourishing yin and tonifying kidney to relieve physical fatigue

目的研究鹿鞭龟甲药对滋阴补肾缓解肾阴虚小鼠体力疲劳作用及机制。方法将70只健康雄性昆明小鼠适应性喂养1周后随机分为空白组、模型组、阳性药组、鹿龟药对低剂量组、鹿龟药对高剂量组、鹿鞭组、龟板组,共7组,每组10只,造模与给药同时进行,共计14天。第14天给药后30分钟测定各组小鼠力竭游泳时间;在第15天摘眼球取血分离血清测定血清中乳酸、尿素氮、睾酮、皮质酮、环腺苷酸(cyclic adenosine monophosphate,cAMP)、环鸟苷酸(cyclic guanosine monophosphate,cGMP)含量;剖取肝脏测定肝糖原含量、肝组织中磷脂酰肌醇3激酶(phosphoinositide3-kinase,PI3K)、蛋白激酶B(protein kinase B,Akt)和糖原合成酶激酶-3(glycogen synthase kinase-3,GSK-3β)蛋白表达。结果与模型组相比,鹿龟药对低剂量组、鹿龟药对高剂量组、鹿鞭组、龟甲组体质量显著升高(P<0.01),力竭游泳时间增加(P<0.05,P<0.01),血乳酸含量降低及血尿素氮含量显著降低(P<0.01),肝糖原含量增加(P<0.05,P<0.01),睾酮含量显著上升(P<0.01),cGMP含量显著升高(P<0.01),cAMP/cGMP值显著下降(P<0.01),肝脏Akt蛋白表达明显增加(P<0.05),肝脏GSK-3β蛋白表达明显降低(P<0.05);鹿龟药对高剂量组及鹿鞭组能显著提高PI3K蛋白表达水平(P<0.05)。结论鹿龟药对可以通过调控PI3K/Akt/GSK-3β通路增加糖原合成,以起到滋阴补肾缓解体力疲劳的作用。

Objective This study aimed to investigate the effect and mechanism of deer penis and tortoise shell on nourishing yin and tonifying kidney to alleviate physical fatigue in mice with kidney yin deficiency.Methods After adaptive feeding for one week,70 healthy male Kunming mice were randomly divided into 7 groups,including the blank group,the kidney yin deficiency model group,the Zuoguiwan medicine group,the low-dose group of deer penis-tortoise shell medicine,the high-dose group of deer penis-tortoise shell medicine,the deer penis group,and the tortoise shell group,with 10 mice in each group.The modeling and administration were conducted simultaneously for a total of 14 days.The exhaustion swimming time was measured 30 minutes after drug administration on the 14th day;On the 15th day,the eyeball was removed and blood was collected to separate serum and measure the levels of lactate,blood urea nitrogen,testosterone,Cortisol,cyclic adenosine monophosphate(cAMP),and cyclic guanosine monophosphate(cGMP).The liver was dissected to measure the content of liver glycogen and the protein expression of phosphoinositide 3 kinase(PI3K),protein kinase B(Akt),and glycogen synthase kinase-3(GSK-3β).Results Compared with the model group,the body weight of the deer penis-tortoise shell drug group and the monotherapy group significantly was increased(P<0.01),the exhaustion swimming time was increased significantly(P<0.05,P<0.01),the blood lactate content was decreased significantly(P<0.01),the blood urea nitrogen content significantly was decreased(P<0.01),the liver glycogen content was increased significantly(P<0.05,P<0.01),the testosterone content was increased significantly(P<0.01),and the cGMP content was increased significantly(P<0.01),cAMP/cGMP ratio was significantly decreased(P<0.01),liver Akt protein expression was significantly increased(P<0.05),and liver GSK-3βprotein expression was significantly decreased(P<0.05),The expression level of PI3K protein in high dose group and deer whip group was significantly increased(P<0.05).Conclusion Deer penis and tortoise shell medicine can increase glycogen synthesis by affecting PI3K/Akt/GSK-3βpathway,thereby nourishing yin and tonifying the kidneys and relieving physical fatigue.