薄型子宫内膜大鼠95%乙醇造模法的实验研究

Thin Endometrium Modeling in Rats with 95%Ethanol:An Experimental Study

目的:探讨薄型子宫内膜大鼠三种造模法的应用价值,探索模型稳定性。方法:采用95%乙醇、脂多糖(LPS)、机械搔刮三种方法建立薄型子宫内膜大鼠模型,术后1周收取子宫内膜组织,HE及Masson染色检测子宫内膜厚度及纤维化面积,免疫组化检测细胞角蛋白CK19及波形蛋白Vimentin的表达以评估子宫内膜细胞的再生情况;通过线粒体凋亡诱导因子2的表达了解子宫内膜细胞线粒体凋亡情况。结果:三种方法均可使大鼠子宫内膜变薄,但光镜下机械组和LPS组子宫内膜结构紊乱,厚薄不均,不能均一地比较子宫内膜厚度,不利于后续实验的开展。与对照组相比,95%乙醇组化学损伤恒定、程度适合,子宫内膜变薄,纤维化面积增大;免疫组化染色95%乙醇组子宫内膜CK19和Vimentin表达均明显较少,线粒体凋亡诱导因子2显著增多。结论:95%乙醇造模法模拟薄型子宫内膜大鼠模型稳定且高效,可用于后续的科学研究。

Objective To explore the application value of three modeling methods for thin endometrium rats and their stabili⁃ty.Methods Three thin endometrium rat models were established using 95%ethanol,lipopolysaccharide(LPS),and me⁃chanical scraping separately.Endometrium tissue was collected one week after surgery.HE staining and Masson staining were performed to detect endometrium thickness and fibrosis area,and immunohistochemistry was used to evaluate the re⁃generation of endometrium cells by detecting cytokeratin 19(CK19)and vimentin.Mitochondrial apoptosis-inducing factor 2 was also analyzed to detect the mitochondrial apoptosis in the endometrium cells.Results All three methods were success⁃ful in thinning the endometrium of rats.However,under light microscopy,the structure of the endometrium in the mechani⁃cal scraping and LPS groups was found to be disordered and uneven,which made it difficult to uniformly compare the endo⁃metrium thickness.In comparison to the control group,the 95%ethanol group showed consistent and appropriate chemical damage,leading to thinned endometrium and increased fibrosis area.Immunohistochemical staining revealed significant re⁃ductions in CK19 and vimentin in the endometrium of the 95%ethanol group,while mitochondrial apoptosis-inducing fac⁃tor 2 was significantly increased.Conclusion Modeling with 95%ethanol was the most stable and efficient method for simu⁃lating the thin endometrium in rats,which can be utilized for subsequent scientific research.