基于非靶向代谢组技术研究不同水力滞留时间对厌氧发酵中代谢产物的影响

Effect of Different Hydraulic Retention Time on Metabolites in Anaerobic Fermentation Based on Non-targeted Metabolomics Technology

采用GC-MS非靶向代谢组学技术,运用主成分分析、正交偏最小二乘分析、聚类分析、差异代谢物代谢通路分析及火山图分析等方法,对厌氧发酵过程中不同水力滞留时间的代谢产物差异性进行了分析。结果表明:共鉴定出已知命名的代谢物369种,其中有机酸、氨基酸及其衍生物和芳香族化合物三类化合物占比最高,占总代谢物的39.57%;通过构建OPLS-DA模型,设置VIP≥1且P<0.05,FC≥2.0和FC≤0.5为筛选阈值,共筛选出差异代谢产物474种,其中已知差异代谢物48种;随着水力滞留时间的缩短,正戊酸、己酸、苯甲酸和3-苯丙酸呈现显著的先下调后上调的现象。综上,研究结果可为厌氧发酵产沼气的代谢调控提供一定的科学依据。

Non-targeted metabolomics technology GC-MS,principal component analysis,orthogonal partial least squares analysis,cluster analysis,differential metabolite metabolic pathway analysis and volcano plot analysis were used to analyze the differences of metabolites in anaerobic fermentation with different hydraulic retention time.The results showed that a total of 369 known named metabolites were identified,of which organic acids,amino acids and derivatives and aromatic compounds accounted for the highest proportion,accounting for 39.57%of the total metabolites.According to the criteria of VIP≥1 and P<0.05,FC≥2.0 and FC≤0.5 in the OPLS-DA model,474 differential metabolites were screened,of which 48 were known.With the shortening of hydraulic retention time,valeric acid,hexanoic acid,benzoic acid and 3-phenylpropionic acid showed a significant decrease first and then increase.In conclusion,the results can provide a scientific basis for the metabolic regulation of biogas production by anaerobic fermentation.