长链非编码RNA活化T细胞核因子非编码基因和长链非编码RNA小分子NF90相关RNA与三阴性乳腺癌预后的相关性分析

Correlation between LncRNA NRON and lncRNA snaR and prognosis of triple negative breast cancer

目的 探究长链非编码RNA(lncRNA)活化T细胞核因子非编码基因(NRON)和lncRNA小分子NF90相关RNA(snaR)与三阴乳腺癌(TNBC)预后的相关性。方法 2018年2月~2020年2月我院收治的TNBC病人100例,术中取其肿瘤组织及癌旁组织,根据3年随访预后情况将其分为预后良好组(72例)与预后不良组(28例)。采用实时定量荧光PCR(RT-qPCR)法检测肿瘤组织与癌旁组织lncRNA NRON、lncRNA snaR表达水平。Pearson法分析TNBC病人肿瘤组织lncRNA NRON与lncRNA snaR的相关性。多因素Cox回归分析TNBC病人预后的影响因素。受试者工作特征(ROC)曲线分析TNBC病人肿瘤组织lncRNA NRON和lncRNA snaR表达水平对预后的预测价值。结果 与癌旁组织相比,TNBC病人肿瘤组织lncRNA NRON表达水平显著下降(1.01±0.10比0.65±0.08,P<0.001),lncRNA snaR表达水平显著上升(1.03±0.13比2.42±0.30,P<0.001)。lncRNA NRON、lncRNA snaR表达水平与TNM分期、肿瘤分化程度有关(P<0.05)。与预后良好组相比,预后不良组TNBC病人肿瘤组织lncRNA NRON表达水平显著下降(0.69±0.09比0.55±0.05,P<0.001),lncRNA snaR表达水平显著上升(2.28±0.26比2.78±0.40,P<0.001)。Pearson法分析显示,TNBC病人肿瘤组织lncRNA NRON与lncRNA snaR表达水平呈负相关(r=-0.617,P<0.001)。多因素Cox回归分析结果显示,lncRNA NRON是TNBC病人预后的保护因素(P<0.05),lncRNA snaR是TNBC病人预后的危险因素(P<0.05)。lncRNA NRON和lncRNA snaR表达水平联合预测TNBC病人预后优于lncRNA snaR单独预测(Z_(二者联合-lncRNA snaR)=3.200,P=0.001)。结论 TNBC病人肿瘤组织中lncRNA NRON表达下调,lncRNA snaR表达上调,二者与TNBC病人预后有关,可能是TNBC预后预测的生物标志物。

Objective To explore the relationship between long non-coding RNA(lncRNA)non-coding repressor of nuclear factor of activated T cells(NRON),long non-coding RNA small NF90-associated RNA(snaR)and the prognosis of triple negative breast cancer(TNBC).Methods From February 2018 to February 2020,100 TNBC patients accepted by our hospital were collected,and their tumor and adjacent tissues were taken during surgery.Based on the 3-year follow-up prognosis,they were separated into a good prognosis group(72 cases)and a poor prognosis group(28 cases).Real-time quantitative fluorescent PCR(RT-qPCR)method was applied to detect the expression levels of lncRNA NRON and lncRNA snaR in tumor tissue and adjacent tissues.Pearson method was applied to analyze the correlation between lncRNA NRON and lncRNA snaR in tumor tissue of TNBC patients.Multivariate Cox regression was applied to analyze the prognostic factors of TNBC patients.Receiver operating characteristic(ROC)curve was applied to analyze the prognostic value of lncRNA NRON and lncRNA snaR expression levels in tumor tissue of TNBC patients.Results Compared with adjacent tissues,the level of lncRNA NRON in tumor tissues of TNBC patients obviously decreased(1.01±0.10 vs 0.65±0.08,P<0.001),while the level of lncRNA snaR obviously increased(1.03±0.13 vs 2.42±0.30,P<0.001).The expression levels of lncRNA NRON and lncRNA snaR were related to TNM staging and tumor differentiation(P<0.05).Compared with the good prognosis group,the level of lncRNA NRON in tumor tissue of TNBC patients in poor prognosis group obviously decreased(0.69±0.09 vs 0.55±0.05,P<0.001),while the level of lncRNA snaR obviously increased(2.28±0.26比2.78±0.40,P<0.001).Pearson method analysis showed that there was a negative correlation between lncRNA NRON and lncRNA snaR expression levels in tumor tissue of TNBC patients(r=-0.617,P<0.001).Multivariate Cox regression analysis showed that lncRNA NRON was a protective factor for the prognosis of TNBC patients(P<0.05),LncRNA snaR was a risk factor for the prognosis of TNBC patients(P<0.05).The combination of lncRNA NRON and lncRNA snaR expression levels in predicting better prognosis in TNBC patients than lncRNA snaR alone(Z combination-lncRNA snaR=3.200,P=0.001).Conclusion The level of lncRNA NRON is down-regulated and lncRNA snaR level is obviously up-regulated in tumor tissues of TNBC patients,they are related to the prognosis of TNBC patients and may be biomarkers for evaluating TNBC prognosis.