白癜风患者外周血单个核细胞CXC趋化因子配体10、CXC趋化因子受体3表达水平及检测意义

Expression and significance of CXC chemokine ligand-10 and CXC chemokine ligand receptor-3 in peripheral blood mononuclear cells of patients with vitiligo

目的:探究白癜风患者外周血单个核细胞(PBMC)中CXC趋化因子配体10(CXCL10)、CXC趋化因子受体3(CXCR3)的表达水平及检测意义。方法:选取收治的白癜风患者120例为观察组,另选取同期性别、年龄相匹配的体检健康者120例为对照组。比较两组PBMC中CXCL10、CXCR3 mRNA表达水平,分析PBMC中CXCL10、CXCR3 mRNA表达与白癜风患者临床特征的关系。统计患者复发情况,并分为复发组(55例)和未复发组(65例)。比较复发组和未复发组患者PBMC中CXCL10/CXCR3 mRNA表达水平。评价PBMC中CXCL10、CXCR3 mRNA对白癜风患者复发风险的预测价值。结果:观察组PBMC中CXCL10及CXCR3 mRNA表达水平高于对照组(均P<0.05)。患者PBMC中CXCL10、CXCR3 mRNA表达水平在不同疾病分期、皮损面积及病程方面比较差异有统计学意义(均P<0.05)。PBMC中CXCL10、CXCR3 mRNA表达与患者疾病分期、皮损面积及病程呈正相关(均P<0.05)。复发组患者PBMC中CXCL10、CXCR3 mRNA表达水平高于未复发组(均P<0.05)。PBMC中CXCL10、CXCR3 mRNA以及两者联合预测白癜风患者复发风险的AUC分别为0.781、0.754、0.934,且两者联合预测的AUC高于各指标单独预测的AUC(均P<0.05)。结论:白癜风患者PBMC中CXCL10及CXCR3 mRNA表达水平升高,与疾病分期、皮损面积、病程有关,且两者联合对白癜风患者复发风险的预测价值较高。

Objective:To investigate the expression levels of CXC chemokine ligand-10(CXCL10)and CXC chemokine ligand receptor-3(CXCR3)in peripheral blood mononuclear cells(PBMC)of patients with vitiligo and their detection significance.Methods:A total of 120 patients with vitiligo were selected as the observation group,and another 120 healthy individuals matched for age and gender from the same period were selected as the control group.The mRNA expression levels of CXCL10 and CXCR3 in PBMC were compared between the two groups,and the relationship between the expression of CXCL10 and CXCR3 mRNA in PBMC and the clinical characteristics of vitiligo patients was analyzed.The recurrence status of patients was recorded,and they were divided into a recurrence group(55 cases)and a non-recurrence group(65 cases).The mRNA expression levels of CXCL10 and CXCR3 in PBMC were compared between the recurrence and non-recurrence groups.The predictive value of CXCL10 and CXCR3 mRNA in PBMC for the risk of recurrence in patients with vitiligo was evaluated.Results:The mRNA expression levels of CXCL10 and CXCR3 in PBMC were higher in the observation group than in the control group(all P<0.05).The expression levels of CXCL10 and CXCR3 mRNA in PBMC differed significantly among different stages of the disease,lesion area and disease duration(all P<0.05).The expression of CXCL10 and CXCR3 mRNA in PBMC was positively correlated with the disease stage,lesion area and disease duration of the patients(all P<0.05).The mRNA expression levels of CXCL10 and CXCR3 in PBMC were higher in the recurrence group than in the non-recurrence group(all P<0.05).The AUC of CXCL10,CXCR3 mRNA and their combination in predicting the recurrence risk of patients with vitiligo were 0.781,0.754 and 0.934,respectively,and the AUC of their combined prediction was higher than that of each index alone(all P<0.05).Conclusion:The mRNA expression levels of CXCL10 and CXCR3 in PBMC are elevated in patients with vitiligo and are associated with disease stage,lesion area and disease duration.The combined detection of both markers has a high predictive value for the risk of recurrence in patients with vitiligo.