清脂化瘀方HPLC-CAD特征图谱建立及6种糖类含量测定

Establishment of HPLC-CAD characteristic chromatograms and content determination of six carbohydrates for Qingzhi Huayu Formula

目的建立清脂化瘀方HPLC-CAD特征图谱,并测定鼠李糖、果糖、甘露醇、D-无水葡萄糖、蔗糖、棉子糖的含量。方法分析采用Dikma Polyamino HILIC色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;体积流量0.8 mL/min;柱温35℃。结果15批样品特征图谱中有14个特征峰相似度大于0.90。6种糖类在各自范围内线性关系良好(r>0.9950),平均加样回收率93.34%~105.64%,RSD 0.5%~2.6%。结论该方法准确可靠,灵敏度高,重复性好,可用于清脂化瘀方糖类的质量控制。

AIM To establish the HPLC-CAD characteristic chromatograms of Qingzhi Huayu Formula,and to determine the contents of rhamnose,fructose,mannitol,D-anhydrous glucose,sucrose and raffinose.METHODS The analysis was performed on a 35℃thermostatic Dikma Polyamino HILIC column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-water flowing at 0.8 mL/min in a gradient elution manner.RESULTS There were 14 common peaks in the characteristic chromatograms of 15 batches of samples with the similarities of more than 0.90.Six carbohydrates showed good linear relationships within their own ranges(r>0.9950),whose average recoveries were 93.34%-105.64%with the RSDs of 0.5%-2.6%.CONCLUSION This accurate,reliable,sensitive and reproducible method can be used for the quality control of carbohydrates in Qingzhi Huayu Formula.