菊粉调节肠道菌群对精神分裂症小鼠情绪认知的影响

Effects of inulin on emotional disorders and cognitive disfunction in schizophrenia mice by regulating gut microbiota

目的研究菊粉对精神分裂症小鼠情绪认知障碍的改善效果。方法将60只雄性C57BL/6J小鼠随机分为4组:1组为正常对照组,其余3组给予MK-801(0.6 mg/kg)连续14天腹腔注射造模后,分别给予菊粉(2 g/kg)、利培酮(0.1 mg/kg)、正常饮水干预6周;监测体重;16S rRNA高通量测序检测小鼠粪便中的差异性菌群;行为学测试(旷场、水迷宫实验)检测小鼠的认知水平;ELISA法检测脑组织多巴胺、5-羟色胺和脑源性神经营养因子水平。结果菊粉干预组小鼠体重反跳性增长较模型组降低(P<0.05);双歧杆菌菌属丰度较模型组增加,阿克曼氏菌属丰度较模型组降低(P<0.05);旷场结果显示菊粉干预组小鼠自主运动总距离、外周距离及抬爪次数较模型组明显增加(P<0.05);水迷宫结果显示菊粉干预组小鼠逃逸潜伏期时间明显缩短(P<0.05),且穿越平台次数较模型组有增加趋势;菊粉干预组脑源性神经营养因子水平增加,5-羟色胺水平降低(P<0.05),多巴胺水平较模型组仅有下降趋势。结论菊粉通过调节肠道菌群影响精神分裂症小鼠的情绪和认知功能障碍。

Objective To study the effect of inulin on improving cognitive impairment in schizophrenia mice by regulating gut microbiota.Methods 60 male C57BL/6J mice were randomly divided into four groups:Group one was normal control group,and the other three groups were given MK-801(0.6mg/kg body weight)by intrabitoneal injection for 14 consecutive days,and then given inulin(2 g/kg body weight),risperidone(0.1 mg/kg body weight)and normal water for 6 weeks,respectively.Body weight was monitored.Behavioral tests,including open field and water maze experiments,were used to detect the cognitive lever of mice.16S rRNA high-throughput sequencing was performed to detect the differential gut microbiota in mouse feces.The changes of neurotransmitters dopamine,5-hydroxytryptamine(5-HT)and brain-derived neurotrophic factor in brain tissue were detected by ELISA.Results In the group of mice with schizophrenia treated with inulin,the rebound weight gain was reduced(P<0.05),and the abundance of Bifidobacterium was increased,while that of Akkermansia was decreased(P<0.05).In the open field test,the total distance of spontaneous movement,the peripheral distance,and the number of paw lifts of mice in the inulin intervention group were significantly higher than those in the model group(P<0.05).In the water maze test,the escape latency time of mice in the inulin intervention group was significantly shorter than that in the model group(P<0.05),and the number of crossings of the platform was trending upwards.The levels of brain-derived neurotrophic factor(BDNF)were increased,while the level of 5-HT was decreased(P<0.05)in the inulin intervention group.The level of dopamine was only trending downwards in the inulin intervention group.Conclusion Inulin can regulate the gut microbiota,which may affect the emotional and cognitive dysfunctions in mice with schizophrenia.