基于稳定氢同位素特征的天然番茄红素鉴别研究

Identification of natural lycopene based on hydrogen isotope characterization

为了研究稳定氢同位素在番茄红素掺假检测领域的应用可行性,该研究利用高温裂解元素分析-稳定同位素比值质谱联用技术建立了测定不同来源番茄红素稳定氢同位素的方法。经验证,前处理后番茄红素样品纯度≥94.14%,前处理过程不会改变番茄红素氢稳定同位素比值的测定;该方法δ2H值重复性标准偏差为1.99‰,再现性标准偏差小于3‰。对13种番茄红素原料及番茄红素标准品的稳定氢同位素比值进行测定,人工合成的番茄红素δ2H值为-29.21‰~-56.22‰,天然来源的番茄红素δ2H值为-225.76‰~-256.98‰,发酵来源的番茄红素δ2H值为-81.35‰~-141.24‰。三者稳定氢同位素比值具有明显差异,该方法可以用于不同来源的番茄红素的鉴别。

This study aimed to investigate the feasibility of the application of stable hydrogen isotopes in the field of lycopene adulteration detection.The method using the technique of temperature conversion/elementary analyzer-stable isotope ratio mass spectrometers(TC/EA-IRMS)was developed for the determination of stable hydrogen isotopes of lycopene from different sources.It was verified that the purity of lycopene samples was≥94.14%after pretreatment,and the pretreatment process would not change the determination of lycopene hydrogen stable isotope ratio.The standard deviation of theδ2 H value of the method was 1.99‰for repeatability and the standard deviation of the reproducibility was less than 3‰.The stable hydrogen isotope ratios of 13 lycopene raw materials and lycopene standards were determined,theδ2 H values of the synthetic lycopene ranged from-29.21‰--56.22‰,theδ2 H values of the lycopene from natural sources ranged from-225.76‰--256.98‰,and theδ2 H values of the lycopene from fermentation sources ranged from-81.35‰--141.24‰.The stabilized hydrogen isotope ratios of the three are significantly different,and the method can be used for the identification of lycopene from different sources.