Treatments of Pollen with Different Solvents Affect the Seed Setting Rate of Rice(Oryza sativa L.)

不同溶剂处理花粉对水稻结实率的影响

This study aimed to investigate the effects of treatments of rice pollen with different solvents on the seed setting rate.Rice pollen was treated with ultrapure water,0.5%dimethyl sulfoxide,ethanol(1%,5%,and 8%),and 0.5%dimethyl sulfoxide+8%ethanol,and the pollen without treatment was taken as the control.Then,crossing was conducted with the treated rice plants as the male parents and plants subjected to emasculation by warm water as the female parents.The seed setting rates of the male and female parents were then recorded.The results showed that the treatments with different solvents significantly decreased the seed setting rates of both male and female parents.The treatment with 1%ethanol resulted in the highest seed setting rates for both male and female parents,while the treatment with 0.5%dimethyl sulfoxide resulted in the lowest seed setting rates for both parents.Although these solvents affected the seed setting rate of rice,they served as intermediate solvents to dissolve chemicals used in the induction of rice haploids.This study layed a technical foundation for subsequent chemical induction of rice haploids.

为探明不同溶剂处理水稻花粉对水稻结实率的影响,以无处理为对照,采用超纯水、0.5%二甲基亚砜、1%乙醇、5%乙醇、8%乙醇和0.5%二甲基亚砜+8%乙醇处理水稻花粉,再以处理过的水稻为父本与温汤杀雄的母本进行杂交,统计母本和父本植株处理过的穗子结实率。结果表明:与对照组相比,不同溶剂处理组的水稻父母本结实率均下降且差异显著。其中1%乙醇处理组的水稻父母本的结实率相对较高,0.5%二甲基亚砜处理组的最低。虽然这些溶剂会影响水稻种子结实率,但是并不影响它们作为中间溶剂溶解诱导化合物。该试验为后续化学诱导水稻单倍体奠定了技术基础。