摘要
为明确骨桥蛋白(OPN)和多房棘球蚴(Echinococcus multilocularis,Em)p38丝裂原活化蛋白激酶(p38MAPK)信号通路在Em发育过程的作用,剖检感染Em 4~6月的长爪沙鼠,提取Em原头节后,分别用不同浓度的p38MAPK抑制剂(SB202190)处理Em原头节,选取合适的SB202190浓度后在体外将原头节分为DMSO组、anti-p38MAPK组、PBS组、OPN组、OPN+anti-p38MAPK组,采用伊红染色、caspase-3试剂盒检测caspase-3、EDU和Hoechst染色、活性氧(ROS)检测探针检测原头节的活性、凋亡和增殖情况。结果显示,抑制Em的SB202190适宜浓度为20μmol/L,SB202190增加Em的伊红染色率、caspase-3水平,减少EDU阳性、ROS水平,OPN可减少Em伊红染色率、caspase-3水平,增加EDU阳性率、ROS水平,且SB202190可逆转OPN对Em的结果,这些结果表明SB202190可抑制Em的活性和增殖、促进凋亡并与浓度正相关,而OPN可促进Em的活性和增殖、抑制凋亡,且SB202190可逆转OPN对Em的作用。研究结果为OPN和p38MAPK信号通路可能成为治疗多房棘球蚴病的新分子靶点提供证据。
To clarify the role of osteopontin(OPN)and Echinococcus multilocularis(Em)p38 mitogen-activated protein kinase(p38MAPK)signaling pathways in Em development,long-clauded gerbils infected with Em for 4-6 months were dissected.After extracting the Em protoscoleces,the Em protoscoleces were treated with different concentrations of a p38MAPK inhibitor(SB202190).After selecting the appropriate concentration of SB202190,the protoscoleces were divided into a DMSO group,an anti-p38MAPK group,a PBS group,an OPN group,and an OPN+anti-p38MAPK group in vitro.Eosin staining and the caspase-3 kit were used to detect caspase-3;EDU and Hoechst staining and the reactive oxygen species(ROS)probe were used to detect the activity,apoptosis,and proliferation of the protoscoleces.The results showed that the appropriate concentration of SB202190 for Em inhibition was 20μmol/L.SB202190 increased Em Eosin staining rate and caspase-3 level,decreased EDU positive level and ROS level,OPN decreased Em Eosin staining rate and caspase-3 level,increased EDU positive rate and ROS level,and OPN decreased Em Eosin staining rate and caspase-3 level.Moreover,SB202190 can reverse the effect of OPN on Em.These results indicate that SB202190 can inhibit the activity and proliferation of Em,promote apoptosis,and be positively correlated with concentration,while OPN can promote the activity and proliferation of Em,inhibit apoptosis,and reverse the effect of OPN on Em.These results provide evidence that OPN and p38MAPK signaling pathways may be new molecular targets for the treatment of alveolar echinococcosis.
作者
徐刚
毛艺
胡帅
葛宇飞
徐志
张玉梦
谢士伟
张宏伟
张示杰
XU Gang;MAO Yi;HU Shuai;GE Yu-fei;XU Zhi;ZHANG Yu-meng;XIE Shi-wei;ZHANG Hong-wei;ZHANG Shi-jie(First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,Xinjiang,832000,China;School of Medicine,Shihezi University,Shihezi,Xinjiang,832000,China)
出处
《动物医学进展》
北大核心
2025年第1期9-15,共7页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(8176120052
81860363)。