心脉灵注射液对培养神经细胞谷氨酸损伤的影响

Effect of Xinmailing Solution on neuronal cell injury induced by glutamate

目的 :观察心脉灵注射液和MK - 80 1对谷氨酸所致的培养神经细胞损伤的作用。方法 :制备培养神经细胞谷氨酸损伤模型 ,测定上清液中一氧化氮和丙二醛的含量 ,同时相差显微镜下观察活细胞的形态改变。结果 :MK - 80 1和心脉灵液可使谷氨酸致神经细胞形态损伤得到减轻 ,并可使培养上清液中增高的一氧化氮和丙二醛含量显著低于谷氨酸组。结论

AIM: To detect the effects of Xinmailing Solution and MK-801 on injury of neuronal cell induced by glutamate. METHODS: Cultured neuronal cell injuried by glutamate was prepared and the content of malondialdehyde and nitrite in cell supernatant was measured. Morphology changes were also observed with discrepancy microscope at the same time. RESULTS: Xinmailing Solution and MK-801 attenuated cell injury induced by glutamate,and inhibited increase in malondialdehyde and nitrite in cell supernatant. CONCLUSION: Xinmailing Solution had a protective effect on neuronal cell at cell level.