肾移植患者手术前后血清sIL-2R检测

Detection of serum soluble interleukin-2 receptor before and after operation in renal transplantations

目的:探讨血清可溶性白细胞介素2受体(sIL-2R)在肾移植急性排斥中的应用价值。方法:采用双抗体夹心ELISA法检测170例肾移植患者手术前后血清sIL-2R浓度。肾移植患者分为肾病组(n=27),慢性肾炎组(n=137),感染组(n=6),另设正常对照组(n=20)。结果:sIL-2R术后较术前明显升高者27例,分别为肾病组12例,慢性肾炎组9例,感染组6例。27例患者术后血清sIL-2R水平显著高于正常对照组(P<0.01),经临床证实为急性排斥者21例,余6例因感染引起。结论:血清sIL-2R浓度可以作为肾移植排斥反应早期诊断一个有实用价值的指标。

Aim:To study the value of soluble interleukin-2 receptor (sIL-2R) in renal transplantation acute rejecti-om. Methods:The level of sIL-2R in serum was determined with double-antibody sandwich ELISA in 170 patients before and after renal transplantation. All the patients were allocated into 4 groups:nephropathy group(n =27) , chronic nephritis group (n = 137) ,infection group (n =6) ,and control group(n =20). ReSUltS;The postoperative level of sIL-2R was higher than those before operation in 27 cases,among which nephropathy group had 12 cases,chronic nephritis group had 9 cases , and infection group had 6 cases. The level of sIL-2R of the 27 cases was higher than that of control group ( P < 0. 01) , among which 21 cases were clinically confirmed as acute rejection, and the other 6 cases attributed to infection. CoPClu-Sion :It is suggested that the level of sIL-2R can be used as an index for early diagnosis of renal rejection in renal transplantation.