Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells

bFGF在人眼组织中的表达及其对晶体上皮细胞增殖的影响(英文)

OBJECTIVE: To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age. METHODS: Enucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry. RESULTS: bFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P

目的 研究碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)在人眼中的表达及对白内障患者人晶体上皮细胞 (humanlensepithelialcells ,HLEC)增殖的影响。方法 取人眼球免疫组化测定bFGF蛋白 ;白内障术中取晶体前囊膜bFGF培养 ,4 8小时后免疫组化染色和JDIAS医用多功能图象分析软件测定增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)阳性面积率。结果 bFGF在晶体及晶体周围组织广泛存在 ;其促白内障患者HLEC增殖作用呈剂量依赖性 ,且与白内障患者年龄呈负相关 (P <0 0 5 )。结论 bFGF促白内障患者术后残留HLEC增殖 ,参与了后发障的形成。