CK34βE12和PSA顺序免疫组化双染色在前列腺病变中的表达

THE EXPRESSION OF PSA AND CK34βE12 IN PROSTATE LESIONS PERFORMED BY IMMUNOENZYMATIC DOUBLE STAINING

目的探索免疫组化前列腺特异性抗原(PSA)、高分子量角蛋白(CK34βE12)双重染色法,并观察二者在前列腺增生性病变、癌前病变、癌及易被误为前列腺癌的精囊腺组织中的表达差异。方法采用PSA、CK34βE12顺序免疫组化双重染色法,选择良性前列腺增生症8例、腺病6例、萎缩后增生4例、基底细胞增生4例、高级别上皮内瘤6例、前列腺癌30例及精囊腺4例,观察染色变化。结果本染色方法稳定、结果分辨清晰。PSA在良性前列腺增生症、腺病、高级别上皮内瘤的分泌上皮、和绝大多数前列腺癌细胞中强表达,在萎缩后增生中不表达或弱表达,基底细胞增生、精囊腺不表达。CK34βE12在前列腺癌中完全缺失,而在增生性病变、癌前病变呈完整或断续状,精囊腺中表达完整。结论CK34βE12和PSA顺序免疫组化双染色在前列腺良、恶性、癌前性病变的诊断和鉴别诊断中是个可行而有用的辅助手段。

Objective The immunoenzymatic double stainin g procedure for PSA and CK34βE12is explored,and different expressive patterns are o bserved in prostate proliferative d isorders,preneoplastic lesions,a denocarcinoma,and seminal vesicle which often misinte rpreted as carcinoma.Methods The sequential immunoenzymatic dou ble staining was employed with two antibodies of PSA a nd CK34βE12to observe the staining patterns in 8cases of benign prostatic hyperp lasia(BPH),6cases of adenosis,4cases of posta trophic hyperplasia,6cases of high grade prostatic intraepithelial neoplasia(HGPIN),4cases of basal cell hyperplasia(BCH),30cases of prostatic adenocarcinoma and 4cases of seminal vesicle.Results The method is stable and shows a good c olour contrast.PSA expression is in tensive in secretary cells of BPH,adenosis,HGPIN and in the majority o f adenocarcinoma,declined or negative in postatrophic hyperplasia,ab sent in BCH and seminal vesicle.CK34βE12staining reveals complete absen ce in adenocarcinoma,a complete or f ragmented layer in prolif-erative,preneoplastic lesions,an d a complete layer in seminal vesicle.Conclusions Sequential immunoenzymatic double staining of CK34βE12and PSA is a feasible and useful ad junctive method in diagnosis as well as differential diagnosis in benign,malignant,preneoplastic l esions of the prostate.