多轮复合诱变选育抗真菌抗生素高产菌株及其发酵条件研究

Complex Mutagensis and Screening of High Productivity Strains of Antifungal Antibiotic and Their Fermentive Conditions

对抗真菌抗生素 Terreic acid- 179M(简称 179M)产生菌黄柄曲霉 ( Aspergillusf lavipes) 进行紫外线、亚硝基胍、紫外线加亚硝基胍多轮复合诱变 ,以对自身次生代谢产物抗性作为筛选策略 ,选育到对自身抗生素抗性提高且发酵相对效价提高到 383%的高产菌株。菌落形态观察发现在 Sabauraud( SBD)培养基上产孢子丰富且形成黄色孢子和黄色菌丝的 179M产量较高。179M最佳发酵温度为 2 8℃ ,培养基最适起始 p H值为

Aspergillus flavipes produced by antifungal antibiotic of Terreic acid 179M(designated as 179M),was treated by UV,NG and UV+NG in sequence.The mutated strains were selected by self secondary metabolites.The microorganism with relative potency of 179M increased to 383% and increasing resistance to 179M were obtained.It was found that the strain 179M has high productivity with abundant yellow spores and hyphas in the medium Sabauraud.The favor temperature to the fermentation of 179M was 28℃ and the favor initial pH value was 6