摘要
目的 :探讨人巨细胞病毒 (humancytomegalovirus ,HCMV)感染昆明小鼠的敏感性 ,建立HCMV致畸的动物模型。方法 :昆明系小鼠 4 0只 ,随机分为两组 :对照组 (8只 )和接种病毒组 (32只 ) ,病毒感染途径为脑内注射。在接种病毒 7,1 5 ,30 ,6 0d后不同时期分别以病理学方法观察动物脑部发育特点 ,以免疫组织化学方法检查病毒抗原 ,PCR方法检测动物脑组织中HCMV基因。结果 :接种HCMVAD1 69株的小鼠脑组织在不同时期发生了病理改变和脑部发育畸形 ,用免疫组织化学方法和PCR方法在脑组织细胞内检获HCMV抗原和基因。结论 :HCMVAD1 69株可引起小鼠脑部发育畸形 。
Objective To determine the sensitivity of mouse to human cytomegalovirus AD 169 strain (HCMV AD 169 ) infection and to establish a stable animal model for developmental disorders. Methods Forty kunmin mice were divided into two groups randomly: the virus inoculated group and the control group. After 7, 15, 30, and 60 days, the mice's brain development was observed by pathological techniques. HCMV antigen was detected by immunohistochemical method and HCMV DNA was analyzed by polymerase chain reaction (PCR). Results The extensive pathological damages and developmental disorders in the brain tissues of infected mice were observed. Meanwhile, the viral antigen and viral DNA were also demonstrated in the brain tissues of HCMV infected mice. Conclusion HCMV can cause brain developmental disorders in mice, which provides a rationale for understanding the mechanism of congenital abnormality by HCMV infection.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第3期243-246,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金资助 (批准号 :3 970 0 0 0 6)
