反相高效液相色谱-化学发光法测定复合维生素片剂中的维生素B_1和B_2

Determination of Thiamine and Riboflavin by Reversed-Phase High Performance L iquid Chromatography with Chemiluminescence Detection

基于水溶性维生素在碱性介质中只有维生素B1(VB1)和维生素B2(VB2)可以被K3Fe(CN)6直接氧化产生化学发光的原理,建立了反相高效液相色谱(RP HPLC)分离柱后化学发光检测VB1和VB2的新方法,并成功应用于复合维生素B片剂中VB1和VB2的测定。该方法测定VB1,VB2的线性范围分别为1 0×10-3～1 0g/L和1 0×10-3～0 1g/L,检出限分别为2×10-4g/L和8×10-4g/L。对1 0×10-2g/LVB1,VB2溶液连续11次测定的相对标准偏差分别为2 8%,0 9%。

Based on the direct chemiluminescence reaction of thiamine (VB1) and riboflavi n (VB2) with hexacyanoferrate (Ⅲ) in sodium hydroxide medium, a novel high pe rformance liquid chromatography (HPLC)-chemiluminescence (CL) detection method for simultaneous determination of VB1 and VB2 was developed. The proposed m ethod has been applied satisfactorily to the determination of VB1 and VB2 in a pharmaceutical preparation. The chemiluminescence intensity was linear with V B1 and VB2 concentrations in the ranges of 10×10-3-10 g/L and 10×10-3-01 g/L, respectively. The limits of detection (LOD) for VB1 and VB2 were 2×10-4 g/L and 8×10-4 g/L, re spectively. The relative standard deviations (RSD) (n=11) were 28% for  1.0×10-2 g/L VB1, and 09% for 10×10-2 g/L VB 2.